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Rationale design of biotinylated antimalarial endoperoxide carbon centered radical prodrugs for applications in proteomics.

Barton, Victoria, Ward, Stephen ORCID: https://orcid.org/0000-0003-2331-3192, Chadwick, James, Hill, Alasdair and O'Neill, Paul M (2010) 'Rationale design of biotinylated antimalarial endoperoxide carbon centered radical prodrugs for applications in proteomics.'. Journal of medicinal chemistry, Vol 53, Issue 11, pp. 4555-4559.

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Abstract

The semisynthetic artemisinin derivatives such as artesunate and artemether, along with the fully synthetic endoperoxide antimalarials (e.g., OZ277, Nature 2004, 430, 900-904), are believed to mediate their antimalarial effects by iron-induced formation of carbon-centered radicals capable of alkylating heme and/or protein. Here, we describe the design and synthesis of a series of biotinylated endoperoxide probe molecules for use in proteomic studies. The target molecules include derivatives of the artemisinin and OZ families, and we demonstrate that these conjugates express nanomolar in vitro activity versus cultured strains of Plasmodium falciparum. We also describe the synthesis of chemically cleavable linked conjugates designed to enable mild elution of labeled proteins during target protein identification.

Item Type: Article
Subjects: QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA.
QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 256 Antimalarials
QV Pharmacology > QV 34 Experimental pharmacology (General)
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1021/jm100201j
Depositing User: Mary Creegan
Date Deposited: 01 Sep 2010 09:33
Last Modified: 06 Feb 2018 13:00
URI: https://archive.lstmed.ac.uk/id/eprint/1016

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