LSTM Home > LSTM Research > LSTM Online Archive

A marker of glutathione S-transferase-mediated resistance to insecticides is associated with higher Plasmodium infection in the African malaria vector Anopheles funestus

Downloads

Downloads per month over past year

Tchouakui, Magellan, Chiang, Mu-Chun, Ndo, Cyrille, Kuicheu, Carine, Amvongo-Adjia, Nathalie, Wondji, Murielle, Tchoupo, Micareme, Kusimo, Michael, Riveron, Jacob ORCID: https://orcid.org/0000-0002-5395-767X and Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673 (2019) 'A marker of glutathione S-transferase-mediated resistance to insecticides is associated with higher Plasmodium infection in the African malaria vector Anopheles funestus'. Scientific Reports, Vol 9, e5772.

[img] Text
revised submitted 080319 225525_2_merged_1552052886.docx - Accepted Version
Available under License Creative Commons Attribution.

Download (480kB)
[img]
Preview
Image
image25.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (53kB) | Preview
[img]
Preview
Image
image24.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (56kB) | Preview
[img]
Preview
Image
image23.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (2kB) | Preview
[img]
Preview
Image
image22.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (1kB) | Preview
[img]
Preview
Image
image21.png - Supplemental Material
Available under License Creative Commons Attribution.

Download (911B) | Preview
[img]
Preview
Image
image20.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (1kB) | Preview
[img]
Preview
Image
image19.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (4kB) | Preview
[img]
Preview
Image
image18.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (2kB) | Preview
[img]
Preview
Image
image17.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (1kB) | Preview
[img]
Preview
Image
image16.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (1kB) | Preview
[img]
Preview
Image
image15.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (9kB) | Preview
[img]
Preview
Image
image14.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (2kB) | Preview
[img]
Preview
Image
image13.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (1kB) | Preview
[img]
Preview
Image
image12.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (10kB) | Preview
[img]
Preview
Image
image11.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (11kB) | Preview
[img]
Preview
Image
image10.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (10kB) | Preview
[img]
Preview
Image
image9.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (13kB) | Preview
[img]
Preview
Image
image8.png - Supplemental Material
Available under License Creative Commons Attribution.

Download (2kB) | Preview
[img]
Preview
Image
image7.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (99kB) | Preview
[img]
Preview
Image
image6.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (47kB) | Preview
[img]
Preview
Image
image5.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (19kB) | Preview
[img]
Preview
Image
image4.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (34kB) | Preview
[img]
Preview
Image
image3.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (7kB) | Preview
[img]
Preview
Image
image2.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (16kB) | Preview
[img]
Preview
Image
image1.jpeg - Supplemental Material
Available under License Creative Commons Attribution.

Download (7kB) | Preview

Abstract

Metabolic resistance to insecticides is threatening malaria control in Africa. However, the extent to which it impacts malaria transmission remains unclear. Here, we investigated the association between a marker of glutathione S-transferase mediated metabolic resistance and Plasmodium infection in field population of Anopheles funestus s.s. in comparison to the A296S-RDL target site mutation. The 119F-GSTe2 resistant allele was present in southern (Obout) (56%) and central (Mibellon) (25%) regions of Cameroon whereas the 296S-RDL resistant allele was detected at 98.5% and 15% respectively. The whole mosquito Plasmodium and sporozoite infection rates were 57% and 14.8% respectively in Obout (n = 508) and 19.7% and 5% in Mibellon (n = 360). No association was found between L119F-GSTe2 genotypes and whole mosquito infection status. However, when analyzing oocyst and sporozoite infection rates separately, the resistant homozygote 119F/F genotype was significantly more associated with Plasmodium infection in Obout than both heterozygote (OR = 2.5; P = 0.012) and homozygote susceptible (L/L119) genotypes (OR = 2.10; P = 0.013). In contrast, homozygote RDL susceptible mosquitoes (A/A296) were associated more frequently with Plasmodium infection than other genotypes (OR = 4; P = 0.03). No additive interaction was found between L119F and A296S. Sequencing of the GSTe2 gene showed no association between the polymorphism of this gene and Plasmodium infection. Glutathione S-transferase metabolic resistance is potentially increasing the vectorial capacity of resistant An. funestus mosquitoes. This could result in a possible exacerbation of malaria transmission in areas of high GSTe2-based metabolic resistance to insecticides.

Item Type: Article
Subjects: QX Parasitology > Protozoa > QX 135 Plasmodia
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1038/s41598-019-42015-1
Depositing User: Stacy Murtagh
Date Deposited: 10 Apr 2019 14:29
Last Modified: 11 Apr 2019 08:35
URI: http://archive.lstmed.ac.uk/id/eprint/10384

Statistics

View details

Actions (login required)

Edit Item Edit Item