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Development of a High-Throughput Cytometric Screen to Identify Anti-Wolbachia Compounds: The Power of Public–Private Partnership

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Clare, Rachel ORCID: https://orcid.org/0000-0002-3945-0530, Clark, Roger, Bardelle, Catherine, Harper, Paul, Collier, Matthew, Johnston, Kelly, Plant, Helen, Plant, Darren, McCall, Eileen, Slatko, Barton E., Cantin, Lindsey, Wu, Bo, Ford, Louise, Murray, David, Rich, Kirsty, Wigglesworth, Mark, Taylor, Mark and Ward, Steve ORCID: https://orcid.org/0000-0003-2331-3192 (2019) 'Development of a High-Throughput Cytometric Screen to Identify Anti-Wolbachia Compounds: The Power of Public–Private Partnership'. SLAS Discovery, Vol 24, Issue 5, pp. 537-547.

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Abstract

The Anti-Wolbachia (A·WOL) consortium at the Liverpool School of Tropical Medicine (LSTM) has partnered with the Global High-Throughput Screening (HTS) Centre at AstraZeneca to create the first anthelmintic HTS for neglected tropical diseases (NTDs). The A·WOL consortium aims to identify novel macrofilaricidal drugs targeting the essential bacterial symbiont (Wolbachia) of the filarial nematodes causing onchocerciasis and lymphatic filariasis. Working in collaboration, we have validated a robust high-throughput assay capable of identifying compounds that selectively kill Wolbachia over the host insect cell. We describe the development and validation process of this complex, phenotypic high-throughput assay and provide an overview of the primary outputs from screening the AstraZeneca library of 1.3 million compounds.

Item Type: Article
Subjects: QW Microbiology and Immunology > Bacteria > QW 150 Proteobacteria. Rickettsiaceae, Wolbachia
QX Parasitology > Helminths. Annelida > QX 301 Filarioidea
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 680 Tropical diseases (General)
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1177/2472555219838341
Depositing User: Stacy Murtagh
Date Deposited: 09 Apr 2019 07:15
Last Modified: 31 May 2019 14:50
URI: http://archive.lstmed.ac.uk/id/eprint/10527

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