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Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus.

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Martins, Fabricio, Wilding, Craig, Isaacs, Alison ORCID: https://orcid.org/0000-0002-2452-3338, Rippon, Emily, Megy, Karine and Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497 (2019) 'Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus.'. Scientific Reports, Vol 9, Issue 1, p. 11406.

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Abstract

Culex quinquefasciatus plays an important role in transmission of vector-borne diseases of public health importance, including lymphatic filariasis (LF), as well as many arboviral diseases. Currently, efforts to tackle C. quinquefasciatus vectored diseases are based on either mass drug administration (MDA) for LF, or insecticide-based interventions. Widespread and intensive insecticide usage has resulted in increased resistance in mosquito vectors, including C. quinquefasciatus. Herein, the transcriptome profile of Ugandan bendiocarb-resistant C. quinquefasciatus was explored to identify candidate genes associated with insecticide resistance. High levels of insecticide resistance were observed for five out of six insecticides tested, with the lowest mortality (0.97%) reported to permethrin, while for DDT, lambdacyhalothrin, bendiocarb and deltamethrin the mortality rate ranged from 1.63-3.29%. Resistance to bendiocarb in exposed mosquitoes was marked, with 2.04% mortality following 1 h exposure and 58.02% after 4 h. Genotyping of the G119S Ace-1 target site mutation detected a highly significant association (p < 0.0001; OR = 25) between resistance and Ace1-119S. However, synergist assays using the P450 inhibitor PBO, or the esterase inhibitor TPP resulted in markedly increased mortality (to ≈80%), suggesting a role of metabolic resistance in the resistance phenotype. Using a novel, custom 60 K whole-transcriptome microarray 16 genes significantly overexpressed in resistant mosquitoes were detected, with the P450 Cyp6z18 showing the highest differential gene expression (>8-fold increase vs unexposed controls). These results provide evidence that bendiocarb resistance in Ugandan C. quinquefasciatus is mediated by both target-site mechanisms and over-expression of detoxification enzymes.

Item Type: Article
Subjects: QX Parasitology > Helminths. Annelida > QX 301 Filarioidea
QX Parasitology > Insects. Other Parasites > QX 530 Culex
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
WH Hemic and Lymphatic Systems > Lymphatic System > WH 700 Lymphatic system. Lymphatic diseases (General)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1038/s41598-019-47850-w
Depositing User: Stacy Murtagh
Date Deposited: 12 Aug 2019 09:20
Last Modified: 16 Sep 2019 09:18
URI: https://archive.lstmed.ac.uk/id/eprint/11421

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