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A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin

Hartkoorn, R. C., Khoo, S., Back, D. J., Tjia, J. F., Waitt, C. J., Chaponda, M., Davies, G., Ardrey, Alison, Ashleigh, Samantha and Ward, Stephen ORCID: https://orcid.org/0000-0003-2331-3192 (2007) 'A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin'. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Vol 857, Issue 1, pp. 76-82.

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Abstract

Rifampicin is active against both intracellular and extracellular Mycobacterium tuberculosis. The ability to measure rifampicin drug concentrations in both plasma and in cells may be useful in evaluating the suitability of dosage regimens for populations and individuals. Here a novel simple. precise and accurate method for the quantification of rifampicin in both cells and plasma is reported. Sample proteins were precipitated with acetonitrile containing the internal standard and then diluted with water. Aliquots of supernatant were then injected into the HPLC-MS system for chromatographic separation and detection. Rifampicin calibration curves encompassed concentrations from 100 to 12,800 ng/mL. Intraand inter-assay precision and accuracy were determined using low, medium and high concentration quality control samples and was found to be within 10% in all cases. Rifampicin concentrations were found to be unaffected by freeze-thaw cycles, but were significantly affected by heat-inactivation (58 degrees C 40 min). This assay was successfully utilised to determine the pharmacokinetic profile of rifampicin in plasma and peripheral blood mononuclear cells (PBMC) in 8 tuberculosis patients receiving rifampicin over an 8 h period. (c) 2007 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: rifampicin hplc-ms plasma cellular liquid-chromatographic determination hiv-infected patients antituberculosis drugs pyrazinamide pharmacokinetics blood
Subjects: QU Biochemistry > Cells and Genetics > QU 300 General works
QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 268 Antitubercular agents. Antitubercular antibiotics
QV Pharmacology > QV 38 Drug action.
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.jchromb.2007.07.005
Depositing User: Ms Julia Martin
Date Deposited: 16 Sep 2010 10:28
Last Modified: 06 Feb 2018 13:01
URI: https://archive.lstmed.ac.uk/id/eprint/1206

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