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In vitro antimalarial susceptibility profile and PRCRT/PFMDR-1 genotypes of Plasmodium falciparum field isolates from Malawi

Nkhoma, Standwell, Molyneux, Malcolm E and Ward, Stephen ORCID: https://orcid.org/0000-0003-2331-3192 (2007) 'In vitro antimalarial susceptibility profile and PRCRT/PFMDR-1 genotypes of Plasmodium falciparum field isolates from Malawi'. American Journal of Tropical Medicine and Hygiene, Vol 76, Issue 6, pp. 1107-1112.

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Abstract

We measured in vitro antimalarial drug susceptibility of 84 Plasmodium falciparum field isolates from Blantyre, Southern Malawi, using the WHO microtest and the lactate dehydrogenase assay. We also genotyped these isolates to investigate whether variation in their absolute drug sensitivity is associated with specific sets of pfcrt and pfmdr-1 mutations harbored by parasites. Our results show that nearly a decade after the withdrawal of chloroquine (CQ) as a first-line antimalarial in Malawi, most isolates are now sensitive to CQ and none is CQ-resistant as predicted by their drug sensitivity phenotype and pfcrt genotype. We also found that these isolates are uniformly sensitive to a range of quinoline-based antimalarials and artemisinin derivatives. These findings reinforce previous reports about a reduction in the proportion of CO-resistant parasites after the withdrawal of CQ in 1993 and pave the way for reassessing the clinical usefulness of CQ, artemisinins and other quinoline-based antimalarials in Malawi.

Item Type: Article
Additional Information: No DOI.
Uncontrolled Keywords: chloroquine resistance drug-resistance cross-resistance east-africa sensitivity invitro malaria mefloquine amodiaquine efficacy
Subjects: QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 256 Antimalarials
QX Parasitology > Protozoa > QX 135 Plasmodia
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Depositing User: Ms Julia Martin
Date Deposited: 19 Oct 2010 09:10
Last Modified: 06 Feb 2018 13:01
URI: https://archive.lstmed.ac.uk/id/eprint/1256

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