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Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia

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Alanazi, Abdullah, Puschendorf, Robert, Alyousif, Mohamed, Al-Khalifa, Mohamed, Alharbi, Samir, Al-Shehri, Zafer, Said, Ashraf, Alanazi, Ibrahim, Al-Mohammed, Hamdan and Alraey, Yasser (2019) 'Molecular Detection of Leishmania spp. in Skin and Blood of Stray Dogs from Endemic Areas of Cutaneous Leishmaniasis in Saudi Arabia'. Iranian Journal of Parasitology, Vol 14, Issue 2, pp. 231-239.

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Abstract

Dogs can act as reservoirs of canine leishmaniasis, caused by Leishmania species. The aims of this study were to determine the prevalence of canine leishmaniasis using a PCR technique among stray dogs living in three provinces of Saudi Arabia, Riyadh, Al-Ahsa Oasis and Al-Qaseem, here the disease is endemic; and to identify and document different Leishmania to species levels
Methods: This cross-sectional investigation was conducted, from Mar 2016 to Apr 2018, in three parts of Saudi Arabia: Central province (Riyadh), Eastern province (Al-Ahsa Oasis) and Al-Qaseem province. Blood samples were collected from 526 dogs; 40 presented cutaneous nodules so were suspected clinically of cutaneous leishmaniasis. Biopsy tissue collections and parasite cultures were performed. A generic kDNA was performed using different primers for Leishmania
differentiation.
Results: All blood samples were negative for Leishmania infantum infection by molecular analysis, though forty dogs had thick cutaneous lesions in different parts of their body. Four dogs’ skin lesions were associated with dermatitis, splenomegaly and lymphadenomegaly. Parasite culture was used to diagnose cutaneous leishmaniasis, identifying 31/40 (77.5%) positive samples. Overall, of 526 samples, the prevalence of L. major and L. tropica was found to be 4% and 1.9%,
respectively. Gender and age had a significant effect on Leishmania prevalence: (P=0.0212 and0.0357), respectively.
Conclusion: This was the first molecular study of dog leishmaniasis from Saudi Arabia of dogs confirmed to have cutaneous leishmaniasis. Further epidemiological and molecular investigations of domestic and wild canine infections with L. major, L. tropica and L. infantum in endemic and nonendemic areas of Saudi Arabia are required, for leishmaniasis control.

Item Type: Article
Subjects: WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 950 Zoonoses (General)
WR Dermatology > Parasitic Skin Diseases > WR 345 Parasitic skin diseases (General or not elsewhere classified)
WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.18502/ijpa.v14i2.1135
Depositing User: Cathy Waldron
Date Deposited: 28 Oct 2019 15:19
Last Modified: 31 Oct 2019 11:02
URI: https://archive.lstmed.ac.uk/id/eprint/12917

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