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Lung fluid immunoglobulin from HIV-infected subjects has impaired opsonic function against pneumococci

Eagan, R., Twigg, H. L., French, Neil, Musaya, J., Day, R. B., Zijlstra, E. E., Tolmie, Helen, Wyler, D., Molyneux, Malcolm E and Gordon, Stephen ORCID: https://orcid.org/0000-0001-6576-1116 (2007) 'Lung fluid immunoglobulin from HIV-infected subjects has impaired opsonic function against pneumococci'. Clinical Infectious Diseases, Vol 44, Issue 12, pp. 1632-1638.

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Abstract

Background. The incidence of pneumococcal pneumonia is greatly increased among human immunodeficiency virus (HIV)-infected subjects, compared with among non-HIV-infected subjects. Lung fluid levels of immunoglobulin G (IgG) specific for pneumococcal capsular polysaccharide are not reduced in HIV-infected subjects; therefore, we examined immunoglobulin subtypes and compared lung fluid IgG opsonic function in HIV-infected subjects with that in healthy subjects.
Methods. Bronchoalveolar lavage (BAL) fluid and serum samples were collected from 23 HIV-infected and 26 uninfected subjects. None of the subjects were receiving highly active antiretroviral therapy, and none had received pneumococcal vaccination. Pneumococcal capsule-specific IgG levels in serum and BAL fluid were measured by enzyme-linked immunosorbent assay, and IgG was concentrated from 40 mL of BAL fluid. Opsonization and opsonophagocytosis of pneumococci with serum, BAL fluid, and BAL IgG were compared between HIV-infected subjects and healthy subjects.
Results. The effect of type 1 pneumococcal capsular polysaccharide - specific IgG in opsonizing of pneumococci was significantly less using both serum and BAL IgG from HIV-infected subjects, compared with serum and BAL IgG from healthy subjects (mean level, 8.9 fluorescence units [95% confidence interval, 8.1-9.7 fluorescence units] vs. 12.1 fluorescence units [95% confidence interval, 9.7-15.2 fluorescence units]; P = .002 for lung BAL IgG). The opsonophagocytosis of pneumococci observed using BAL IgG from HIV-infected subjects was significantly less than that observed using BAL IgG from healthy subjects (37 fluorescence units per ng of IgG [95% confidence interval, 25-53 fluorescence units per ng of IgG] vs. 127 fluorescence units per ng of IgG [95% confidence interval, 109-145 fluorescence units per ng of IgG]; P < .001).
Conclusion. HIV infection is associated with decreased antipneumococcal opsonic function in BAL fluid and serum.

Item Type: Article
Uncontrolled Keywords: immunodeficiency-virus-infection linked-immunosorbent-assay streptococcus-pneumoniae polysaccharide vaccine alveolar macrophages malawian adults immune-response ugandan adults antibody disease
Subjects: WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections
WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503.5 Complications
Digital Object Identifer (DOI): https://doi.org/10.1086/518133
Depositing User: Ms Julia Martin
Date Deposited: 06 Sep 2010 12:44
Last Modified: 15 Jun 2018 14:46
URI: https://archive.lstmed.ac.uk/id/eprint/1352

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