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Field evaluation of DNA detection of human filarial and malaria parasites using mosquito excreta/feces

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Minetti, Corrado ORCID: https://orcid.org/0000-0001-7862-4874, Pilotte, Nils, Zulch, Michael, Canelas, Tiago, Tettevi, Edward J., Veriegh, B. D., Osei-Atweneboana, Mike Yaw, Williams, Stephen A. and Reimer, Lisa ORCID: https://orcid.org/0000-0002-9711-4981 (2020) 'Field evaluation of DNA detection of human filarial and malaria parasites using mosquito excreta/feces'. PLoS Neglected Tropical Diseases, Vol 14, Issue 4, e0008175.

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Abstract

Background
We recently developed a superhydrophobic cone-based method for the collection of mosquito excreta/feces (E/F) for the molecular xenomonitoring of vector-borne parasites showing higher throughput compared to the traditional approach. To test its field applicability, we used this platform to detect the presence of filarial and malaria parasites in two villages of Ghana and compared results to those for detection in mosquito carcasses and human blood.

Methodology and principal findings
We compared the molecular detection of three parasites (Wuchereria bancrofti, Plasmodium falciparum and Mansonella perstans) in mosquito E/F, mosquito carcasses and human blood collected from the same households in two villages in the Savannah Region of the country. We successfully detected the parasite DNA in mosquito E/F from indoor resting mosquitoes, including W. bancrofti which had a very low community prevalence (2.5-3.8%). Detection in the E/F samples was concordant with detection in insect whole carcasses and human blood, and laboratory tests showed that the risk of mosquito carcass cross-contamination with positive excreta when insects are held together in the device is low.

Conclusions
Our approach to collect and test mosquito E/F successfully detected a variety of parasites at varying prevalence in the human population under field conditions, including a pathogen (M. perstans) which is not transmitted by mosquitoes. The method shows promise for further development and applicability for the early detection and surveillance of a variety of pathogens carried in human blood.

Item Type: Article
Subjects: QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA.
QX Parasitology > QX 4 General works
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1371/journal.pntd.0008175
Depositing User: Mel Finley
Date Deposited: 09 Apr 2020 15:09
Last Modified: 09 Apr 2020 15:44
URI: https://archive.lstmed.ac.uk/id/eprint/13852

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