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Cas9-mediated gene-editing in the malaria mosquito Anopheles stephensi by ReMOT Control

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Macias, Vanessa M, McKeand, Sage, Chaverra-Rodriguez, Duverney, Hughes, Grant ORCID: https://orcid.org/0000-0002-7567-7185, Fazekas, Aniko, Pujhari, Sujit, Jasinskiene, Nijole, James, Anthony A and Rasgon, Jason L (2020) 'Cas9-mediated gene-editing in the malaria mosquito Anopheles stephensi by ReMOT Control'. G3, Vol 10, Issue 3.

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Abstract

Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by Anopheles mosquitoes.
CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially Anopheles) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control (Receptor-mediated Ovary Transduction of Cargo) technique to
deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos. In Anopheles, ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to Anopheles opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and
establishes the flexibility of ReMOT Control for diverse mosquito species.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 450 General Works
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1534/g3.120.401133
Depositing User: Mel Finley
Date Deposited: 24 Mar 2020 11:34
Last Modified: 24 Mar 2020 11:34
URI: https://archive.lstmed.ac.uk/id/eprint/13925

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