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Trypanosoma brucei colonises the tsetse gut via an immature peritrophic matrix in the proventriculus

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Rose, Clair ORCID: https://orcid.org/0000-0001-7782-5359, Casas Sanchez, Aitor, Dyer, Naomi, SolorzanoGonzalez, Carla, Beckett, Alison J., Middlehurst, Ben, Marcello, Marco, Haines, Lee ORCID: https://orcid.org/0000-0001-8821-6479, Lisack, Jaime, Engstler, Markus, Lehane, Mike, Prior, Ian A. and Acosta-Serrano, Alvaro ORCID: https://orcid.org/0000-0002-2576-7959 (2020) 'Trypanosoma brucei colonises the tsetse gut via an immature peritrophic matrix in the proventriculus'. Nature Microbiology. (In Press)

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Abstract

The peritrophic matrix (PM) of haematophagus insects is a chitinous structure that surrounds the bloodmeal, forming a protective barrier against oral pathogens and abrasive particles. To establish an infection in the tsetse midgut, Trypanosoma brucei must colonise the ectoperitrophic space (ES), located between the PM and gut epithelium. Although unproven, it is generally accepted that trypanosomes reach the ES by directly penetrating the PM in the anterior midgut. Here we revisited this event by employing novel fluorescence and electron
microscopy methodologies and found that instead, trypanosomes reach the ES via the newly secreted PM in the tsetse proventriculus. Within this model, parasites colonising the proventriculus can either migrate to the ES or become trapped within PM layers forming cysts that move along the entire gut as the PM gets remodelled. Early proventricular colonisation appears to be promoted by unidentified factors in trypanosome-infected blood, resulting in higher salivary gland infections and potentially increasing parasite transmission.

Item Type: Article
Subjects: QW Microbiology and Immunology > QW 52 Physiology and chemistry of microorganisms. Metabolism.
QX Parasitology > QX 4 General works
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
Faculty: Department: Biological Sciences > Vector Biology Department
Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1038/s41564-020-0707-z
Depositing User: Cathy Waldron
Date Deposited: 22 Apr 2020 16:09
Last Modified: 24 Apr 2020 09:17
URI: https://archive.lstmed.ac.uk/id/eprint/14144

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