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The whole blood phagocytosis assay: a clinically relevant test of neutrophil function and dysfunction in community-acquired pneumonia

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Reiné, Jesús, Rylance, Jamie ORCID: https://orcid.org/0000-0002-2323-3611, Ferreira, Daniela ORCID: https://orcid.org/0000-0002-0594-0902, Pennington, Shaun ORCID: https://orcid.org/0000-0002-7160-6275, Welters, Ingeborg, Parker, Robert and Morton, Ben ORCID: https://orcid.org/0000-0002-6164-2854 (2020) 'The whole blood phagocytosis assay: a clinically relevant test of neutrophil function and dysfunction in community-acquired pneumonia'. BMC Notes, Vol 13, Issue 203.

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Abstract

Objective: To refine and validate a neutrophil function assay with clinical relevance for patients with communityacquired
pneumonia (CAP).
Design: Two phase cross-sectional study to standardise and refine the assay in blood from healthy volunteers and
test neutrophil phagocytic function in hospital patients with CAP.
Participants: Phase one: Healthy adult volunteers (n = 30). Phase two: Critical care patients with severe CAP (n = 16),
ward-level patients with moderate CAP (n = 15) and respiratory outpatients (no acute disease, n = 15).
Results: Our full standard operating procedure for the assay is provided. Patients with severe CAP had significantly
decreased neutrophil function compared to moderate severity disease (median phagocytic index 2.8 vs. 18.0,
p = 0.014). Moderate severity pneumonia neutrophil function was significantly higher than control samples (median
18.0 vs. 1.6, p = 0.015). There was no significant difference between critical care and control neutrophil function
(median 2.8 vs. 1.6, p = 0.752).
Conclusions: Our whole blood neutrophil assay is simple, reproducible and clinically relevant. Changes in neutrophil
function measured in this pneumonia cohort is in agreement with previous studies. The assay has potential to be
used to identify individuals for clinical trials of immunomodulatory therapies, to risk-stratify patients with pneumonia,
and to refine our understanding of ‘normal’ neutrophil function in infection.
Keywords: Neutrophils, Sepsis, Flow cytometry, Phagocytosis, Pneumonia

Item Type: Article
Subjects: QW Microbiology and Immunology > Immune Responses > QW 640 Agglutination. Precipitation
WC Communicable Diseases > WC 20 Research (General)
WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 202 Pneumonia (General or not elsewhere classified)
WF Respiratory System > WF 140 Diseases of the respiratory system (General)
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Biological Sciences > Department of Tropical Disease Biology
Clinical Sciences & International Health > International Public Health Department
Digital Object Identifer (DOI): https://doi.org/10.1186/s13104-020-05034-0
Depositing User: Catherine Molloy
Date Deposited: 22 Apr 2020 15:31
Last Modified: 22 Apr 2020 15:31
URI: https://archive.lstmed.ac.uk/id/eprint/14293

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