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A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

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Simoni, Alekos, Hammond, Andrew M., beaghton, Andrea K.Beaghton, Galizi, Roberto, Taxiarchi, Chrysanthi, Kyrou, Kyros, Meacci, Dario, Gribble, Matthew, Morselli, Giulia, Burt, Austin, Nolan, Tony ORCID: https://orcid.org/0000-0002-2982-8333 and Crisanti, Andrea (2020) 'A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae'. Nature Biotechnology. (In Press)

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Abstract

Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce unisex populations that eventually collapse, but the underlying molecular mechanisms are unknown. We report a male-biased sex-distorter gene drive (SDGD) in the human malaria vector Anopheles gambiae. We induced super-Mendelian inheritance of the X-chromosome-shredding I-PpoI nuclease by coupling this to a CRISPR-based gene drive inserted into a conserved sequence of the doublesex (dsx) gene. In modeling of invasion dynamics, SDGD was predicted to have a quicker impact on female mosquito populations than previously developed gene drives targeting female fertility. The SDGD at the dsx locus led to a male-only population from a 2.5% starting allelic frequency in 10–14 generations, with population collapse and no selection for resistance. Our results support the use of SDGD for malaria vector control.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1038/s41587-020-0508-1
Depositing User: Stacy Murtagh
Date Deposited: 26 May 2020 12:53
Last Modified: 28 May 2020 10:28
URI: https://archive.lstmed.ac.uk/id/eprint/14486

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