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Are Plasmodium falciparum parasites present in peripheral blood genetically the same as those sequestered in the tissues?


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Dembo, E. G., Phiri, H. T., Montgomery, Jacqui, Molyneux, Malcolm E and Rogerson, S. J. (2006) 'Are Plasmodium falciparum parasites present in peripheral blood genetically the same as those sequestered in the tissues?'. American Journal of Tropical Medicine and Hygiene, Vol 74, Issue 5, pp. 730-732.

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Since quinine does not inhibit the growth of Plasmodium falciparum ring stages or mature schizonts, parasites may continue to emerge from sequestration sites after starting treatment. We used polymerase chain reaction amplification of P. falciparum merozoite surface protein 1 (MSP-1) and MSP-2 alleles to distinguish genotypes infecting 58 children with severe malaria. To examine changes in parasite populations in peripheral blood over time, we compared changes in number and spectrum of genotypes in samples on admission to a hospital to those obtained up to 24 hours later. Thirty-four children lost genotypes, 21 retained genotypes, and 3 gained an extra P. falciparum genotype at one locus but not the other. The lack of novel genotypes emerging suggests that among children with severe malaria the dominant clones sequestered in deep organs are usually the same as those in peripheral circulation.

Item Type: Article
Uncontrolled Keywords: t papua-new-guinea malaria populations children infections complexity dynamics quinine drugs area
Subjects: QX Parasitology > Protozoa > QX 135 Plasmodia
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Depositing User: Sarah Lewis-Newton
Date Deposited: 19 Apr 2011 15:12
Last Modified: 17 Jul 2020 10:57


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