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Anopheles gambiae P450 reductase is highly expressed in oenocytes and in vivo knockdown increases permethrin susceptibility

Lycett, Gareth ORCID: https://orcid.org/0000-0002-2422-053X, McLaughlin, L. A., Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247, Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173, Kafatos, F. C., Loukeris, T. G. and Paine, Mark ORCID: https://orcid.org/0000-0003-2061-7713 (2006) 'Anopheles gambiae P450 reductase is highly expressed in oenocytes and in vivo knockdown increases permethrin susceptibility'. Insect Molecular Biology, Vol 15, Issue 3, pp. 321-327.

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Abstract

We describe an in vivo model for investigation of detoxification mechanisms of the mosquito Anopheles gambiae, important for the development of malaria control programmes. Cytochrome P450s are involved in metabolic insecticide resistance and require NADPH cytochrome P450 reductase (CPR) to function. Here we demonstrate that the major sites of adult mosquito CPR expression are oenocytes, mid-gut epithelia and head appendages. High CPR expression was also evident in Drosophila oenocytes indicating a general functional role in these insect cells. RNAi mediated knockdown drastically reduced CPR expression in oenocytes, and to a lesser extent in mid-gut epithelia; the head was unaffected. These flies showed enhanced sensitivity to permethrin, demonstrating a key role for abdominal/mid-gut P450s in pyrethroid metabolism, aiding the development of insecticides.

Item Type: Article
Uncontrolled Keywords: insecticide resistance rnai mosquito pyrethroid detoxification drosophila-melanogaster house-fly insecticide resistance plasmodium-berghei cytochrome-p450 system gene ecdysone cockroach cloning
Subjects: QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1111/j.1365-2586.2006.00647.x
Depositing User: Sarah Lewis-Newton
Date Deposited: 14 Feb 2011 16:59
Last Modified: 22 Aug 2019 11:03
URI: https://archive.lstmed.ac.uk/id/eprint/1539

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