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Molecular studies of Anopheles culicifacies (Diptera : Culicidae) in Sri Lanka: Sibling species B and E show sequence identity at multiple loci

Surendran, S. N., Hawkes, Nicola J., Steven, Andrew, Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 and Ramasamy, R. (2006) 'Molecular studies of Anopheles culicifacies (Diptera : Culicidae) in Sri Lanka: Sibling species B and E show sequence identity at multiple loci'. European Journal of Entomology, Vol 103, Issue 1, pp. 233-237.

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Abstract

The anomaly that Anopheles culicifacies (Diptera: Culicidae) Species B is a major vector of malaria in Sri Lanka, but a non-vector in India, has been noted for several years. In 1999, a Y chromosome dimorphism associated with Plasmodium vivax infectivity within the Indian A. culicifacies species B suggested that this was itself a complex of two sibling species, B and E. A recent cytogenetic analysis shows the sympatric presence of these sibling species in Sri Lanka, a situation similar to that reported from nearby Rameshwaram Island, India. Species E, with a submetacentric Y chromosome, is a more effective vector of P. vivax than species B with an acrocentric Y chromosome. Larval karyotyping, however, is time-consuming and labour-intensive. Recently, the development of a PCR-RFLP assay distinguishing species B and E of A. culicifacies from India, based oil differences in one region of the cytochrome oxidase subunit II (COII) gene, was reported. Here we show that whilst this diagnostic approach reveals polymorphism in Sri Lankan A. culicifacies, this variation is not correlated with Y chromosome karyotype. Hence this assay will not be useful for distinguishing Species B and E in Sri Lanka. Further, we found no difference between the sequences of Sri Lankan specimens in any of three other regions (ITS2, D3 region of 28S rDNA, and guanylate cyclase intron) often Used for species discrimination.

Item Type: Article
Additional Information: Free PDF available online
Uncontrolled Keywords: anopheles culicifacies species b species e y-chromosome dimorphism cytochrome oxidase ii its2 28s rdna complex diptera gambiae members identification
Subjects: QU Biochemistry > Genetics > QU 475 Genetic processes
QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics.
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WB Practice of Medicine > Medical Climatology > WB 710 Diseases of geographic areas
Faculty: Department: Groups (2002 - 2012) > Vector Group
Depositing User: Sarah Lewis-Newton
Date Deposited: 14 Feb 2011 10:36
Last Modified: 06 Feb 2018 13:02
URI: http://archive.lstmed.ac.uk/id/eprint/1604

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