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Adenosine in the venoms from viperinae snakes of the genus Bitis: Identification and quantitation using LC/MS and CE/MS

Graham, R. L. J., McClean, S., O'Kane, E. J., Theakston, R.David G. and Shaw, C. (2005) 'Adenosine in the venoms from viperinae snakes of the genus Bitis: Identification and quantitation using LC/MS and CE/MS'. Biochemical and Biophysical Research Communications, Vol 333, Issue 1, pp. 88-94.

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Abstract

Snake venoms are rich sources of toxic proteins and small molecules. This study was directed at molecules of molecular mass below 1 kDa. Thirty different venoms, of either neurotoxic or haemorrhagic type, were fractionated using size-exclusion chromatography. Only venoms of the Puff adder (Bitis arietans), Gaboon viper (Bitis gabonica), and Rhinoceros viper (Bitis nasicornis) exhibited large absorbance peaks at gimel(280nm) in the total volume range of the chromatographic column indicating the presence of abundant low molecular mass material. Analysis of fractions containing this material using both HPLC and capillary electrophoresis interfaced with electrospray ion-trap mass spectrometry unequivocally established that the bioactive nucleoside, adenosine, was the major component. The concentrations of adenosine found (Puff adder-97.7 x 10(-6) mol L-1; Gaboon viper-28.0 x 10(-6) mol L-1; and Rhinoceros viper-56.8 x 10(-6) mol L-1) were above those required to activate all known sub-types of adenosine receptors. Adenosine may thus act at the site of envenomation causing local vasodilatation and may play a role in the subsequent systemic hypotension observed. (c) 2005 Elsevier Inc. All rights reserved.

Item Type: Article
Uncontrolled Keywords: adenosine snake venom mass spectrometry angiotensin-converting enzyme gaboon viper mast-cells receptors bradykinin purines circulation hypotension inhibitors gabonica
Subjects: WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 400 General works
WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.bbrc.2005.05.077
Depositing User: Sarah Lewis-Newton
Date Deposited: 12 Oct 2011 15:41
Last Modified: 06 Feb 2018 13:02
URI: http://archive.lstmed.ac.uk/id/eprint/1858

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