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Evidence to support two conspecific cytological races of Anopheles aconitus in Thailand

Junkum, A., Komalamisra, N., Jitpakdi, A., Jariyapan, N., Min, G. S., Park, M. H., Cho, K. H., Somboon, P., Bates, Paul and Choochote, W. (2005) 'Evidence to support two conspecific cytological races of Anopheles aconitus in Thailand'. Journal of Vector Ecology, Vol 30, Issue 2, pp. 213-224.

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Abstract

Iso-female lines (isolines) of Anopheles aconitus collected from Mae Hong Son, Phet Buri, and Chiang Mai Provinces were successfully identified to karyotypic forms. The results of identification revealed that An. aconitus Form B (X-1, X-2, Y-2) was obtained from four and 48 isolines in Phet Buri and Chiang Mai Provinces, respectively, and Form C (X-1 X-2 Y-3) was recovered from three and 41 isolines in Mae Hong Son and Chiang Mai Provinces, respectively. When comparing band to band on the same arm of ovarian nurse cell polytene chromosomes of An. aconitus Form B (Phet Buri: four isolines) and C (Mae Hong Son: three isolines, Chiang Mai: 20 isolines) to the standard chromosome mapping of An. aconitus Form B (Chiang Mai: 20 isolines), no major chromosomal rearrangements that related to the karyotype variations were demonstrated. The investigations on allelic frequencies of 4th stage larvae and adult females of three (Form C: Mae Hong Son), four (Form B: Phet Buri), 41 (Form C: Chiang Mai) and 48 (Form B: Chiang Mai) isolines suggested that An. aconitus Form B and C of all strains have similar allelic frequencies. This was observed at 10 isoenzymes 16 loci in 4th stage larvae, and 11 isoenzymes 13 loci in adult females. Hybridization tests among the four laboratory-raised isolines of An. aconitus Form B (Chiang Mai and Phet Buri) and C (Chiang Mai and Mae Hong Son) were employed by induced copulation. The results of crosses indicated that they were genetically compatible, providing viable progeny and completely synaptic salivary gland polytene chromosomes. The complete sequences of rDNA internal-transcribed spacer two (ITS2) and partial sequences of mitochondrial cytochrome c oxidase subunit I and II (COI and COII) from genomic DNA of 12 isolines of An. aconitus Form B and C were identified. Total sequence lengths (ITS2+COI+COII) of An. aconitus isolines varied from 1550bp to 1556bp. Conspecific relationships between the twoAn. aconitus forms were well supported by low values of intraspecific distances (ranged from 0.1% to 1.0%) and genetic differentiation (d(xy) : 0.0 1322) between the two forms. Based on evidence of no pre- and post-mating isolations, and nearly identical of DNA sequences of ITS2, COI and COII regions between An. aconitus Form B and C, we conclude that they are conspecific cytological races in the Thai population.

Item Type: Article
Uncontrolled Keywords: anopheles aconitus isoenzyme hybridization internal transcribed spacer 2 cytochrome c oxidase subunit i and ii subgenus cellia diptera population-genetic-evidence dirus complex culicifacies complex metaphase karyotypes chromosomal evidence malaria vectors southeast-asia culicidae identification
Subjects: QU Biochemistry > Genetics > QU 450 General Works
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Depositing User: Ms Julia Martin
Date Deposited: 26 Jul 2011 09:53
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/1928

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