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Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test

Salimi-Bejestani, M. R., McGarry, J.W., Felstead, S., Ortiz, P., Akca, A. and Williams, Diana J.L. (2005) 'Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test'. Research in Veterinary Science, Vol 78, Issue 2, pp. 177-181.

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Abstract

An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: fasciola hepatica; cattle; immunodiagnosis; elisa linked-immunosorbent-assay; quantitative diagnosis; bovine fascioliasis; cattle; sheep infections; immunodiagnosis; immunoassay; challenge; responses
Subjects: QX Parasitology > QX 4 General works
Faculty: Department: Groups (2002 - 2012) > Veterinary Parasitology Group (2002-2008)
Digital Object Identifer (DOI): https://doi.org/10.1016/j.rvsc.2004.08.005
Depositing User: Ms Julia Martin
Date Deposited: 05 Dec 2011 11:55
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/1957

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