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Antigenic relationships and relative immunogenicities of isolated metalloproteinases from Echis ocellatus venom

Howes, J.M., Theakston, R.David G. and Laing, Gavin (2005) 'Antigenic relationships and relative immunogenicities of isolated metalloproteinases from Echis ocellatus venom'. Toxicon, Vol 45, Issue 5, pp. 677-680.

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Abstract

The antigenic relationship between snake venom metalloproteinases (SVMPs) was analysed using rabbit antisera raised against the native forms of two SVMPs purified from Echis ocellatus venom. Using enzyme-linked immunosorbent assay (ELISA), western blotting and two-dimensional SDS-PAGE, our findings show that antibodies raised against EoVMP1, a nonhaemorrhagic class P-I 24 kDa SVMP, and EoVMP2, a haemorrhagic class P-III 56 kDa SVMP, demonstrate cross-reactivities which relate to the domain hierarchy observed in class P-I to P-III/IV SVMPs. A third 65 kDa P-III metalloproteinase (designated EoVMP3) was also isolated from E. ocellatus venom using hydrophobic interaction, size exclusion and anion exchange chromatography. In comparative immunoassays, EoVMP2 and EoVMP3 bound strongly to the commercial monovalent ovine Fab fragment antivenom EchiTAb (TM) (raised against the same venom), but EoVMP1 showed no cross-reactivity. This could indicate that antivenoms may lack antibodies to potentially important venom components. (c) 2005 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: echis venom metalloproteinases immunoreactivity immunoassay antivenom snake-venom pathogenesis antivenom nigeria africa
Subjects: QV Pharmacology > Toxicology > General Toxicology > QV 601 Antidotes and other therapeutic measures
QV Pharmacology > Toxicology > General Toxicology > QV 602 Detection of poisons. Tests. Laboratory manuals. Technique
QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids
WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 400 General works
WD Disorders of Systemic, Metabolic or Environmental Origin, etc > Animal Poisons > WD 410 Reptiles
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.toxicon.2005.01.010
Depositing User: Sarah Lewis-Newton
Date Deposited: 17 Oct 2011 15:36
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/1961

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