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Field evaluation of a recombinant glutathione S-transferase-based pyrethroid quantification assay

Enayati, Ahmad Ali, Lengeler, C., Erlanger, T. and Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 (2005) 'Field evaluation of a recombinant glutathione S-transferase-based pyrethroid quantification assay'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 99, Issue 5, pp. 369-378.

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Abstract

A recombinant glutathione S-transferase (GST)-based pyrethroid quantification assay was field-tested in Ifakara, Tanzania. Initial laboratory tests suggested that all reagents used in the assay should be sufficiently stable for field use, provided that domestic refrigeration facilities were available. Insecticide-impregnated bednets were collected from a region where a social marketing programme was in progress. A total of 100 bednets were collected and the assay plus standard HPLC analysis was performed on the residues extracted from four replicate areas of each net. Insecticide residue estimations for assays performed on white and pale green bednet samples were accurate when compared with residue analysis by HPLC. However, for dark green or blue bednets, there was no correlation between the GST-based assay and HPLC pyrethroid quantification results. The assay failure with the dark coloured nets was caused by the extraction of the dyes along with the insecticide, which subsequently interfered with the GST assay. When the same samples were analysed by HPLC, the dyes were separated from the insecticide by reverse phase column chromatography and hence did not affect the results. (c) 2004 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: malaria glutathione s-transferase insecticide quantification field assay field validation tanzania linked-immunosorbent-assay experimental hut trials treated bed nets anopheles-gambiae mosquito nets malaria control permethrin insecticide bednets tanzania
Subjects: QU Biochemistry > Enzymes > QU 135 Enzymes
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.trstmh.2004.09.009
Depositing User: Martin Chapman
Date Deposited: 06 Sep 2011 12:57
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/1966

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