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Salivary gland of Toxorhynchites splendens Wiedemann (Diptera : Culicidae): Ultrastructural morphology and electrophoretic protein profiles

Jariyapan, N., Choochote, W., Jitpakdi, A. and Bates, Paul (2004) 'Salivary gland of Toxorhynchites splendens Wiedemann (Diptera : Culicidae): Ultrastructural morphology and electrophoretic protein profiles'. Journal of Medical Entomology, Vol 41, Issue 4, pp. 569-574.

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Abstract

The salivary glands of male and female Toxorhynchites splendens have the same morphology, and they are paired organs lying on either side of the esophagus. Each gland is composed of two identical tubular lobes, joined together at the end of the proximal region. In the gland, a salivary duct extends through the length of each lobe. The general cellular architecture of the salivary gland of this mosquito is unique. No secretory cavity was found in any cell, and the salivary materials are secreted from long microvilli and collect in a periductal space surrounding the duct. In addition, a number of mitochondria, rough endoplasmic reticulum, and a very large nucleus were observed, suggesting a high energy requirement for producing the salivary proteins involved in sugar feeding. The size of the gland is approximate to50 mum in diameter and 1.5 mm in length. These dimensions correlate with high protein content of these salivary glands (2.88 +/- 0.14 mug/gland pair). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the electrophoretic protein profiles of the male and female salivary glands were identical. No dominant major proteins were found. Compared with Aedes and Anopheles mosquitoes, the protein profile of T splendens was similar to that observed in the males of these other species but different to that shown by the females, thus making T splendens an excellent organism for studying the biochemistry of sugar feeding in mosquitoes.

Item Type: Article
Uncontrolled Keywords: toxorhynchites splendens salivary gland ultrastructure sodium dodecyl sulfate-polyacrylamide gel electrophoresis vector anopheles-gambiae aedes-aegypti malaria vector vaccine candidate live mosquitos dengue sialome stephensi monkeys
Subjects: QX Parasitology > Insects. Other Parasites > QX 505 Diptera
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1603/0022-2585-41.4.569
Depositing User: Sarah Lewis-Newton
Date Deposited: 17 Feb 2012 15:00
Last Modified: 06 Feb 2018 13:03
URI: https://archive.lstmed.ac.uk/id/eprint/2182

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