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The role of salivary nitrophorins in the ingestion of blood by the triatomine bug Rhodnius prolixus (Reduviidae: Triatominae)

Araujo, R. N., Soares, A. C., Paim, R. M. M., Gontijo, N. F., Gontijo, A. F., Lehane, Mike and Pereira, M. H. (2009) 'The role of salivary nitrophorins in the ingestion of blood by the triatomine bug Rhodnius prolixus (Reduviidae: Triatominae)'. Insect Biochemistry and Molecular Biology, Vol 39, Issue 2, pp. 83-89.

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Abstract

To assist haematophagy, Rhodnius prolixus produces several bioactive molecules in its saliva which it injects into the host skin. The most abundant of these molecules are the nitrophorins (NPs). In this work, we reduced the expression of NPI-4 in the saliva of R. prolixus by RNAi and evaluated the subsequent feeding performance of the bugs using the cibarial pump electromyogram either on the dorsal skin or on the tail vein of the mice. NPs salivary mRNA was reduced by > 99% in comparison to controls. Saliva from knockdown nymphs also presented 82% less haemproteins while the total protein was not reduced. Knockdown nymphs feeding on the skin had lower ingestion rates mainly due to the longer cumulative probing time and lower cibarial pump frequency. Another difference was that knockdown insects bit similar to 5 times more. No differences were observed between groups fed on the tail vein. When the feeding sites were compared, nymphs fed on the tail vein had higher effective ingestion rates. These findings endorse the importance of the NPs for the ability of bugs to complete the meal in a short total contact time with a low number of bites, decreasing the perception of the insect by the host. (c) 2008 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: rhodnius prolixus saliva nitrophorin rnai feeding performance vector electromyogram nitric-oxide antihemostatic components heme-proteins probing time brasiliensis hemiptera purification heteroptera anopheles mosquitos
Subjects: QX Parasitology > Insects. Other Parasites > QX 503 Hemiptera (e.g., Bedbugs)
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 705 Trypanosomiasis
QY Clinical Pathology > Blood. Blood Chemistry > QY 450 Blood chemistry
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ibmb.2008.10.002
Depositing User: Users 183 not found.
Date Deposited: 10 Jun 2010 15:10
Last Modified: 06 Feb 2018 12:59
URI: https://archive.lstmed.ac.uk/id/eprint/224

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