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Evaluation of a urinary antigen-based latex agglutination test in the diagnosis of kala-azar in eastern Nepal

Rijal, S., Boelaert, M., Regmi, S., Karki, B. M. S., Jacquet, D., Singh, R., Chance, Michael, Chappuis, F., Hommel, Marcel, Desjeux, P., Van der Stuyft, P., Le Ray, D. and Koirala, S. (2004) 'Evaluation of a urinary antigen-based latex agglutination test in the diagnosis of kala-azar in eastern Nepal'. Tropical Medicine & International Health, Vol 9, Issue 6, pp. 724-729.

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Abstract

BACKGROUND We evaluated the diagnostic accuracy as well as the reproducibility of the urine latex agglutination test 'KAtex' in the diagnosis of kala-azar in patients recruited at a tertiary care centre in Dharan, Nepal, between November 2000 and January 2002.
METHODS All patients presenting with fever of 2 weeks or more and splenomegaly were consecutively enrolled. Bone marrow and - if negative - spleen aspirates were examined for Leishmania donovani. Serum and urine samples were taken in duplicate for the Direct Agglutination Test (DAT) and KAtex. The reference laboratory determined sensitivity and specificity of KAtex. Reproducibility between both laboratories was assessed.
RESULTS KAtex was performed on urine from 155 parasitologically confirmed kala-azar and 77 non-kala-azar cases (parasitology and DAT-negative). KAtex showed a sensitivity of 47.7% (74/155, 95% CI: 39.7-55.9) and a specificity of 98.7% (76/77, 95% CI: 93.0-100.0). Reproducibility of KAtex showed a kappa of 0.684 (P < 0.001, n = 232).
CONCLUSION KAtex evaluation showed high specificity, low sensitivity and moderate reproducibility. A urine test for kala-azar could become a real breakthrough in kala-azar management if its reproducibility and sensitivity could be further improved.

Item Type: Article
Uncontrolled Keywords: visceral leishmaniasis sensitivity and specificity diagnostic accuracy urine antigen detection test nepal indian visceral leishmaniasis dipstick test strip-test field rk39
Subjects: QY Clinical Pathology > Diagnostic Tests > QY 250 Immunodiagnostic tests
WB Practice of Medicine > Diagnosis > General Diagnosis > WB 200 Physical diagnosis (General)
WB Practice of Medicine > Medical Climatology > WB 710 Diseases of geographic areas
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1111/j.1365-3156.2004.01251.x
Depositing User: Sarah Lewis-Newton
Date Deposited: 28 Feb 2012 11:34
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/2253

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