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An adult-specific CYP6P450 gene is overexpressed in a pyrethroid-resistant strain of the malaria vector, Anopheles gambiae

Nikou, Dimitra, Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 and Hemingway, Janet ORCID: https://orcid.org/0000-0002-3200-7173 (2003) 'An adult-specific CYP6P450 gene is overexpressed in a pyrethroid-resistant strain of the malaria vector, Anopheles gambiae'. Gene, Vol 318, pp. 91-102.

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Abstract

Many malaria control programmes are based on insecticide application as adulticides, often in the form of pyrethroid-impregnated bed nets. However, the efficacy of this control measure can be reduced by genetic changes in vector insecticide susceptibility. Pyrethroid resistance has been detected in the major African malaria vector, Anopheles gambiae, and has been attributed to a combination of target site insensitivity and increased oxidative metabolism of the insecticide, catalysed by cytochrome P450s. An adult-specific cytochrome P450 monooxygenase 6 (CYP6) P450 gene, CYP6Z1, located within a large cluster of cytochrome P450 genes in chromosome arm 3R of An. gambiae, is expressed approximately 11-fold higher in males and 4.5-fold in females from a pyrethroid-resistant strain than in a susceptible strain from the same geographical area. In both strains, CYP6Z1 expression is higher in males than females. Southern blot analysis discounted gene amplification as a cause of this overexpression. The isolation of An. gambiae cDNAs encoding cytochrome b(5) and nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH)-cytochrome P450 reductase cDNAs is also reported. (C) 2003 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Insecticide resistance; Monooygenases; Mosquito; Cytochrome b5; NADPH–cytochrome P450 reductase
Subjects: QU Biochemistry > Genetics > QU 500 Genetic phenomena
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/s0378-1119(03)00763-7
Depositing User: Ms Julia Martin
Date Deposited: 16 Sep 2011 10:40
Last Modified: 06 Feb 2018 13:03
URI: http://archive.lstmed.ac.uk/id/eprint/2316

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