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Nasal immunization of mice with Lactobacillus casei expressing the pneumococcal surface protein C primes the immune system and decreased pneumococcal nasopharyngeal colonization in mice

de Lucia Hernani, Marilia, Duarte Ferreira, Patricia Cristina, Ferreira, Daniela ORCID: https://orcid.org/0000-0001-7730-9477, Miyaji, Eliane Namie, Ho, Paulo Lee and Sarno Oliveira, Maria Leonor (2011) 'Nasal immunization of mice with Lactobacillus casei expressing the pneumococcal surface protein C primes the immune system and decreased pneumococcal nasopharyngeal colonization in mice'. FEMS Immunology and Medical Microbiology, Vol 62, pp. 263-272.

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Abstract

Streptococcus pneumoniae colonizes the upper respiratory tract of healthy individuals,from where it can be transmitted to the community. Occasionally, bacteria invade
sterile niches, causing diseases. The pneumococcal surface protein C (PspC) is a virulence factor that is important during colonization and the systemic phases of the diseases. Here, we have evaluated the effect of nasal or sublingual immunization of mice with Lactobacillus casei expressing PspC, as well as prime-boosting protocols
using recombinant PspC, on nasopharyngeal pneumococcal colonization. None of the protocols tested was able to elicit significant levels of anti-PspC antibodies before
challenge. However, a significant decrease in pneumococcal recovery from the nasopharynx was observed in animals immunized through the nasal route with L.casei-PspC. Immune responses evaluated after colonization challenge in this group of mice were characterized by an increase in mucosal anti-PspC immunoglobulin A (IgA) 5 days later, a time point in which the pneumococcal loads were already low. A
negative correlation between the concentrations of anti-PspC IgA and pneumococcal recovery from the nasopharynx was observed, with animals with the lowest colonization levels having higher IgA concentrations. These results show that nasal immunization with L. casei-PspC primes the immune system of mice, prompting faster immune responses that result in a decrease in pneumococcal colonization.

Item Type: Article
Uncontrolled Keywords: Lactobacillus casei PspC pneumococcal colonization
Subjects: WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 204 Pneumococcal pneumonia. Staphylococcal pneumonia
WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 210 Streptococcal infections (General or not elsewhere classified)
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Digital Object Identifer (DOI): https://doi.org/10.1111/j.1574-695X.2011.00809.x
Depositing User: Users 43 not found.
Date Deposited: 17 Nov 2011 16:50
Last Modified: 06 Feb 2018 13:04
URI: https://archive.lstmed.ac.uk/id/eprint/2437

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