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Detection of antileishmanial IgG antibodies and Leishmania-specific antigens in sera from Yemeni patients with visceral leishmaniasis

Azazy, A. A., Nasher, S. T., Ishaq, A., Bin-Muhanna, M. and Chance, Michael (2003) 'Detection of antileishmanial IgG antibodies and Leishmania-specific antigens in sera from Yemeni patients with visceral leishmaniasis'. Annals of Tropical Medicine and Parasitology, Vol 97, Issue 7, pp. 689-695.

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Abstract

As part of an attempt to develop an effective method for the serodiagnosis of visceral leishmaniasis (VL), the sera from 43 Yemeni cases of the disease were screened both for antileishmanial IgG antibodies and for Leishmiania-specific antigens. Indirect ELISA and capture ELISA were used to test each serum for the antibodies and antigens, respectively. Sera from patients with diseases other than leishmaniasis (29 for the antibody-detection assays and 42 for the antigen-detection) and from apparently healthy volunteers were also tested. For each type of assay, the threshold for seropositivity, was set three standard deviations above the mean absorbance value for the sera from the healthy volunteers. Thirty-seven of the 43 VL sera were found positive for Leishmania-specific IgG antibodies and 37 were found positive for Leishmania-specific antigens. The sensitivities of the antibody- and antigen-detection assays were both therefore 86%. The overall specificity of the antibody-detection ELISA (67%) was, however, slightly higher than that of the capture-ELISA (64%).

Item Type: Article
Subjects: QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 575 Antibodies
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis
WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1179/000349803225002481
Depositing User: Sarah Lewis-Newton
Date Deposited: 07 Nov 2012 10:53
Last Modified: 06 Feb 2018 13:04
URI: https://archive.lstmed.ac.uk/id/eprint/2498

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