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Do pollutants affect insecticide-driven gene selection in mosquitoes? Experimental evidence from transcriptomics

Poupardin, Rodolphe, Riaz, Muhammad Asam, Jones, Christopher, Chandor-Proust, Alexia, Reynaud, Stéphane and David, Jean-Philippe (2012) 'Do pollutants affect insecticide-driven gene selection in mosquitoes? Experimental evidence from transcriptomics'. Aquatic Toxicology, Vol 114-5, pp. 49-57.

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Abstract

The control of mosquitoes transmitting infectious diseases relies mainly on the use of chemical insecticides. However, the emergence of insecticide resistance threatens mosquito control programs. Until now, most research efforts have been focused on elucidating resistance mechanisms caused by insecticide treatments. Less attention has been paid to the impact of the mosquito chemical environment on insecticide-driven selection mechanisms. Here the mosquito Aedes aegypti was used as a model species to conduct laboratory experiments combining the exposure of mosquito larvae to a sub-lethal concentration of xenobiotics and their selection with the insecticide permethrin. After 10 generations, bioassays and a transcriptome profiling with a 15k microarray were performed comparatively on all strains. The three selected strains showed a small but significant increase of permethrin resistance compared to the susceptible parental strain. Microarray analysis revealed that the transcription of many genes was altered by insecticide selection. Exposing larvae to sub-lethal concentrations of the pollutant fluoranthene or the insecticide permethrin prior to selection at each generation affected the selection of several genes, including those involved in detoxification, transport and cell metabolism. Genes potentially involved in permethrin resistance and cross-responses between xenobiotics and insecticide were identified. The present study investigated for the first time the impact of the presence of pollutants in mosquito environment on insecticide-driven selection mechanisms. Our results revealed that mosquitoes exposed to xenobiotics show a different adaptive response to insecticide selection pressure. This suggests that insect chemical environment can shape the long-term selection of metabolic mechanisms leading to insecticide resistance.

Item Type: Article
Subjects: QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance.
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 525 Aedes
QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
WC Communicable Diseases > Virus Diseases > Infectious Mononucleosis. Arbovirus Infections > WC 528 Dengue
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.aquatox.2012.02.001
Depositing User: Users 183 not found.
Date Deposited: 09 Mar 2012 14:22
Last Modified: 06 Feb 2018 13:04
URI: http://archive.lstmed.ac.uk/id/eprint/2809

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