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Genetic diversity of Ascaris in southwestern Uganda.

Betson, Martha, Nejsum, Peter, Llewellyn-Hughes, Julia, Griffin, Claire, Atuhaire, Aaron, Arinaitwe, Moses, Adriko, Moses, Ruggiana, Andrew, Turyakira, Grace, Kabatereine, Narcis B and Stothard, J. Russell ORCID: https://orcid.org/0000-0002-9370-3420 (2012) 'Genetic diversity of Ascaris in southwestern Uganda.'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 106, Issue 2, pp. 75-83.

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Abstract

Despite the common occurrence of ascariasis in southwestern Uganda, helminth control in the region has been limited. To gain further insights into the genetic diversity of Ascaris in this area, a parasitological survey in mothers (n=41) and children (n=74) living in two villages, Habutobere and Musezero, was carried out. Adult Ascaris worms were collected from infected individuals by chemo-expulsion using pyrantel pamoate treatment. Genetic diversity within these worms was assessed by inspection of DNA sequence variation in a mitochondrial marker and length polymorphism at microsatellite loci. Overall prevalence of ascariasis was 42.5% in mothers and 30.4% in their children and a total of 98 worms was examined from 18 hosts. Sequence analysis of a portion of the mitochondrial cytochrome c oxidase subunit 1 gene revealed 19 different haplotypes, 13 of which had not been previously encountered. Microsatellite analysis using eight loci provided evidence for high gene flow between worm populations from the two villages but comparing these worms with others obtained in a prior study on Unguja, Zanzibar, confirmed little genetic exchange and mixing of worm populations between the two areas. By adding to our understanding of the genetic diversity of Ascaris in Africa, this study provides useful information for monitoring changes in parasite population structure in the face of ongoing and future control.

Item Type: Article
Uncontrolled Keywords: Ascaris Evolution Cytochrome c oxidase subunit 1 Microsatellite Population genetics Uganda
Subjects: QX Parasitology > Helminths. Annelida > QX 203 Nematoda
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 870 Ascariasis
Digital Object Identifer (DOI): https://doi.org/10.1016/j.trstmh.2011.10.011
Depositing User: Mary Creegan
Date Deposited: 18 May 2012 15:22
Last Modified: 27 Sep 2018 10:34
URI: https://archive.lstmed.ac.uk/id/eprint/2839

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