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Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major

Jamjoom, M.B., Ashford, R.W., Bates, Paul, Kemp, S. J. and Noyes, H. A. (2002) 'Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major'. Molecular Ecology Notes, Vol 2, Issue 2, pp. 104-106.

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Abstract

Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QX Parasitology > Protozoa > QX 70 Mastigophora. (e.g., Giardia. Trichomonas. Trypanosoma. Leishmania)
Faculty: Department: Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1046/j.1471-8286.2002.00161.x
Depositing User: Lynn Roberts-Maloney
Date Deposited: 19 Jul 2013 12:20
Last Modified: 06 Feb 2018 13:05
URI: https://archive.lstmed.ac.uk/id/eprint/2942

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