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Carriage of potentially pathogenic Escherichia coli in chickens

Kariuki, S., Gilks, C.F., Kimari, J., Muyodi, J., Getty, B. and Hart, C. Anthony (2002) 'Carriage of potentially pathogenic Escherichia coli in chickens'. Avian Diseases, Vol 46, Issue 3, pp. 721-724.

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Abstract

DNA-DNA hybridization, cultured cell lines, and transmission electron microscopy were used to study pathogenicity traits of 64 Escherichia coli isolated from apparently healthy chickens from 18 small-scale farms in Thika District, Kenya. A total of 39 (60.9%) isolates hybridized with the eae gene probe for enteropathogenic E. coli (EPEC) whereas another 16 (25%) hybridized with the lt and st gene probes and were categorized as enterotoxigenic E. coli. Electron microscopic examination of the eae probe-positive E coli cultures with the HT-2919A cell line confirmed that they were able to attach intimately and produced effacement typical of EPEC. In addition, negative stain electron microscopy showed that the EPEC strains produced pili that have previously been associated with increased virulence of E. coli infections in chickens. This study has also demonstrated that apparently healthy chickens may carry enteropathogenic E. coli strains.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QU Biochemistry > Proteins. Amino Acids. Peptides > QU 58.5 DNA.
QW Microbiology and Immunology > QW 50 Bacteria (General). Bacteriology. Archaea
WC Communicable Diseases > Infection. Bacterial Infections > Enteric Infections > WC 290 Escherichia coli infections
Faculty: Department: Groups (2002 - 2012) > Child & Reproductive Health Group
Digital Object Identifer (DOI): https://doi.org/10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2
Depositing User: Lynn Roberts-Maloney
Date Deposited: 18 Oct 2013 13:35
Last Modified: 06 Feb 2018 13:05
URI: http://archive.lstmed.ac.uk/id/eprint/2948

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