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Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Daborn, Phillip J., Lumb, Christopher, Harrop, Thomas W.R., Blasetti, Alex, Pasricha, Shivani, Morin, Shai, Mitchell, Sara N., Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497, Müller, Pie and Batterham, Philip (2012) 'Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests'. Insect Biochemistry and Molecular Biology, Vol 42, Issue 12, pp. 918-924.

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Abstract

Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insect
Drosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferring
metabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (aE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes,expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed.

Item Type: Article
Subjects: QW Microbiology and Immunology > Immune Responses > QW 700 Infection. Mechanisms of infection and resistance.
QX Parasitology > Arthropods > QX 460 Arthropods
QX Parasitology > Insects. Other Parasites > QX 500 Insects
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ibmb.2012.09.003
Depositing User: Users 183 not found.
Date Deposited: 02 Jan 2013 15:10
Last Modified: 06 Feb 2018 13:05
URI: https://archive.lstmed.ac.uk/id/eprint/3080

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