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In vitro activity of antimicrobial agents against the endosymbiont Wolbachia pipientis

Hermans, P. G., Hart, C. Anthony and Trees, Alexander J. (2001) 'In vitro activity of antimicrobial agents against the endosymbiont Wolbachia pipientis'. Journal of Antimicrobial Chemotherapy, Vol 47, Issue 5, pp. 659-663.

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Abstract

Arthropod-transmitted (filarial) nematodes are important causes of disease in humans in tropical countries, yet no safe drug appropriate for mass delivery kills the adult worms. However, most filarial nematodes contain rickettsia-like bacteria of the genus Wolbachia, and related bacteria also occur in insects. There is increasing evidence that these bacteria have significant functions in the biology of filarial nematodes, They are thus important targets in the search for antifilarial drugs and experiments in animals and humans have suggested that antibiotic therapy has potential in treating filarial infections. To optimize future clinical trials there is a need for a fast and simple in vitro drug screen to compare drug efficacies against Wolbachia, In the absence of Wolbachia-infected nematode cell lines, we have utilized an Aedes albopictus insect cell line, naturally infected with Wolbachia, to test the activity of antimicrobial agents. Of the five antibiotics tested, doxycycline, oxytetracycline and rifampicin showed good activity (MICs of 0.0625, 4 and 0.0625 mg/L, respectively) whereas ciprofloxacin and penicillin were shown to have no effect.

Item Type: Article
Subjects: QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 253 Anthelmintics
QV Pharmacology > Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents > QV 254 Antiprotozoal agents (General)
QV Pharmacology > Anti-Bacterial Agents. Tissue Extracts > QV 350 Anti-bacterial agents (General or not elsewhere classified)
QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
QW Microbiology and Immunology > QW 52 Physiology and chemistry of microorganisms. Metabolism.
QX Parasitology > Helminths. Annelida > QX 301 Filarioidea
Faculty: Department: Pre 2002
Digital Object Identifer (DOI): https://doi.org/10.1093/jac/47.5.659
Depositing User: Martin Chapman
Date Deposited: 16 Jul 2013 10:49
Last Modified: 06 Feb 2018 13:05
URI: http://archive.lstmed.ac.uk/id/eprint/3164

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