LSTM Home > LSTM Research > LSTM Online Archive

Expression and secretion of a larval-specific chitinase (family 18 glycosyl hydrolase) by the infective stages of the parasitic nematode, Onchocerca volvulus

Wu, Yang, Egerton, Gillian, Underwood, Anthony P., Sakuda, S. and Bianco, Albert E. (2001) 'Expression and secretion of a larval-specific chitinase (family 18 glycosyl hydrolase) by the infective stages of the parasitic nematode, Onchocerca volvulus'. Journal of Biological Chemistry, Vol 276, Issue 45, pp. 42557-42564.

Full text not available from this repository.

Abstract

A recently reported chitinase gene, expressed in the infective, third-stage (L3) larvae of the human filarial parasite Onchocerca volvulus, belongs to the family 18 glycosyl hydrolases and has been designated Ov-chi-1. The gene product of Ov-chi-1 is chitinolytic. Allosamidin ablates activity of the native enzyme in a dose-dependent manner but did not significantly inhibit the moulting of L3 larvae. Mono-specific antibodies were used to characterize Ov-CHI-1 as a 60-kDa protein expressed almost exclusively in L3 stages. Immunoelectron microscopy showed that Ov-CHI-1 expression is initiated in late L2 larvae and increases markedly in infective, L3 larvae. It is synthesized exclusively in the glandular esophagus and stored within discrete secretory granules. Secretion occurs through de-granulation during post-infective development, and the primary route of transport appears to be via the pseudo-coelom. An orthologue of Ov-chi-1 was detected in Caenorhabditis elegans by BLAST analysis. It is constitutively expressed at a low level and is overexpressed in dauer larvae and embryonated eggs. It is chitinolytic. We conclude that Ov-CHI-1 is a highly stage-specific enzyme that may have a role in infectivity of the parasite, aiding escape from the vector or participating in early post-infective migration and/or development. The identification of an orthologue in C. elegans opens the way for further studies into the biological function(s) of this intriguing parasite product.

Item Type: Article
Subjects: QU Biochemistry > Enzymes > QU 136 Hydrolases
QU Biochemistry > Genetics > QU 470 Genetic structures
QU Biochemistry > Genetics > QU 475 Genetic processes
QX Parasitology > Helminths. Annelida > QX 203 Nematoda
Faculty: Department: Pre 2002
Digital Object Identifer (DOI): https://doi.org/10.1074/jbc.M103479200
Depositing User: Lynn Roberts-Maloney
Date Deposited: 19 Feb 2014 09:47
Last Modified: 06 Feb 2018 13:05
URI: https://archive.lstmed.ac.uk/id/eprint/3208

Statistics

View details

Actions (login required)

Edit Item Edit Item