LSTM Home > LSTM Research > LSTM Online Archive

The characterization of the Phlebotomus papatasi transcriptome

Abrudan, J., Ramalho-Ortigão, M., O'Neil, S., Stayback, G., Wadsworth, M., Bernard, M., Shoue, D., Emrich, S., Lawyer, P., Kamhawi, S., Rowton, E. D., Lehane, Mike, Bates, Paul, Valenzeula, J. G., Tomlinson, C., Appelbaum, E., Moeller, D., Thiesing, B., Dillon, Rod, Clifton, S., Lobo, N. F., Wilson, R. K., Collins, F. H. and McDowell, M. A. (2013) 'The characterization of the Phlebotomus papatasi transcriptome'. Insect Molecular Biology, Vol 22, Issue 2, pp. 211-232.

Full text not available from this repository.

Abstract

As important vectors of human disease, phlebotomine sand flies are of global significance to human health, transmitting several emerging and re-emerging infectious diseases. The most devastating of the sand fly transmitted infections are the leishmaniases, causing significant mortality and morbidity in both the Old and New World. Here we present the first global transcriptome analysis of the Old World vector of cutaneous leishmaniasis, Phlebotomus papatasi (Scopoli) and compare this transcriptome to that of the New World vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed using pooled mRNA from Phlebotomus papatasi larvae, pupae, adult males and females fed sugar, blood, or blood infected with Leishmania major. A total of 47 615 generated sequences was cleaned and assembled into 17 120 unique transcripts. Of the assembled sequences, 50% (8837 sequences) were classified using Gene Ontology (GO) terms. This collection of transcripts is comprehensive, as demonstrated by the high number of different GO categories. An in-depth analysis revealed 245 sequences with putative homology to proteins involved in blood and sugar digestion, immune response and peritrophic matrix formation. Twelve of the novel genes, including one trypsin, two peptidoglycan recognition proteins (PGRP) and nine chymotrypsins, have a higher expression level during larval stages. Two novel chymotrypsins and one novel PGRP are abundantly expressed upon blood feeding. This study will greatly improve the available genomic resources for P. papatasi and will provide essential information for annotation of the full genome.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 500 Genetic phenomena
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 715 Visceral leishmaniasis
WR Dermatology > Parasitic Skin Diseases > WR 350 Tropical diseases of the skin. Mucocutaneous leishmaniasis. Leishmaniasis
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1111/imb.12015
Depositing User: Samantha Sheldrake
Date Deposited: 17 Jul 2013 10:21
Last Modified: 06 Feb 2018 13:06
URI: http://archive.lstmed.ac.uk/id/eprint/3431

Statistics

View details

Actions (login required)

Edit Item Edit Item