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A Novel, Multi-Parallel, Real-Time Polymerase Chain Reaction Approach for Eight Gastrointestinal Parasites Provides Improved Diagnostic Capabilities to Resource-Limited At-Risk Populations

Mejia, R., Vicuna, Y., Broncano, N., Sandoval, C., Vaca, M., Chico, M., Cooper, Philip and Nutman, T. B. (2013) 'A Novel, Multi-Parallel, Real-Time Polymerase Chain Reaction Approach for Eight Gastrointestinal Parasites Provides Improved Diagnostic Capabilities to Resource-Limited At-Risk Populations'. American Journal of Tropical Medicine and Hygiene, Vol 88, Issue 6, pp. 1041-1047.

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Abstract

Diagnosis of gastrointestinal parasites has traditionally relied on stool microscopy, which has low diagnostic sensitivity and specificity. We have developed a novel, rapid, high-throughput quantitative multi-parallel real-time polymerase chain reaction (qPCR) platform. Species-specific primers/probes were used for eight common gastrointestinal parasite pathogens: Ascaris lumbricoides, Necator americanus, Ancylostoma duodenale, Giardia lamblia, Cryptosporidium spp., Entamoeba histolytica, Trichuris trichiura, and Strongyloides stercoralis. Stool samples from 400 13-month-old children in rural Ecuador were analyzed and the qPCR was compared with a standard direct wet mount slide for stool microscopy, as were 125 8–14-year-old children before and after anthelmintic treatment. The qPCR showed higher detection rates for all parasites compared with direct microscopy, Ascaris (7.0% versus 5.5%) and for Giardia (31.5% versus 5.8%). Using an enhanced DNA extraction method, we were able to detect T. trichiura DNA. These assays will be useful to refine treatment options for affected populations, ultimately leading to better health outcomes.

Item Type: Article
Subjects: QX Parasitology > Protozoa > QX 123 Apicomplexa
QX Parasitology > Helminths. Annelida > QX 207 Trichuroidea
QX Parasitology > Helminths. Annelida > QX 243 Strongyloidea
QX Parasitology > Helminths. Annelida > QX 277 Ascaroidea
QX Parasitology > Protozoa > QX 70 Mastigophora. (e.g., Giardia. Trichomonas. Trypanosoma. Leishmania)
WB Practice of Medicine > Diagnosis > General Diagnosis > WB 141 General works
WI Digestive System > WI 140 Diseases (General)
WS Pediatrics > Diseases of Children and Adolescents > By System > WS 310 Digestive system
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.4269/ajtmh.12-0726
Depositing User: Martin Chapman
Date Deposited: 14 Jan 2014 15:37
Last Modified: 06 Feb 2018 13:06
URI: http://archive.lstmed.ac.uk/id/eprint/3559

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