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A molecular phylogeny of mosquitoes in the Anopheles barbirostris Subgroup reveals cryptic species: Implications for identification of disease vectors

Paredes-Esquivel, C., Donnelly, Martin ORCID: https://orcid.org/0000-0001-5218-1497, Harbach, R. E. and Townson, Harold (2009) 'A molecular phylogeny of mosquitoes in the Anopheles barbirostris Subgroup reveals cryptic species: Implications for identification of disease vectors'. Molecular Phylogenetics and Evolution, Vol 50, Issue 1, pp. 141-151.

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Abstract

The Barbirostris Subgroup of the genus Anopheles includes six mosquito species that are almost identical in adult morphology, but differ in their roles in the transmission of malaria and filariasis within Southeast Asia. The lack of robust, diagnostic morphological characters in adults has contributed to extensive misidentification of the species. Mosquitoes were collected from localities in Thailand and Indonesia, with an emphasis on specimens identified in the field as An. barbirostris and An. campestris. A 754 bp COI mitochondrial gene fragment was sequenced from 136 specimens and the rDNA ITS2 region (c.1600-1800 bp) from 51 specimens. Phylogenetic analyzes based on Bayesian methods, distance measures and Maximum-parsimony produced five clades (I-V) that are congruent between the nuclear and mitochondrial data sets. Based on adult female morphology, it is deduced that three of these clades, I-III, are members of the Barbirostris Complex whereas Clade V is An. campestris. The identity of Clade IV is as yet unknown. Using a haplotype network analysis, Clade III was found to have a star-like genealogy, suggesting population expansion. There were no shared haplotypes between clades. In Afrotropical anopheline mosquitoes, speciation has been linked to the expansion of human populations and the development of agriculture. We postulate that the radiation of species within the Barbirostris Subgroup in Southeast Asia may similarly be linked to human population expansion and the agrarian revolution. The development of a propensity for feeding on the blood of humans in some species of the Subgroup would have led to the transmission of malaria protozoa and filarial nematodes. (C) 2008 Elsevier Inc. All rights reserved.

Item Type: Article
Uncontrolled Keywords: anopheles barbirostris anopheles campestris bayesian analysis phylogeny mtdna coi rrna its2 southeast asia thailand indonesia vector identification polymerase-chain-reaction ribosomal DNA population-structure mitochondrial-DNA southeast-asia diptera-culicidae nucleotide substitution transcribed spacer malaria vectors dirus mosquitos
Subjects: WC Communicable Diseases > Tropical and Parasitic Diseases > WC 680 Tropical diseases (General)
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 755 Epidemiology
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WB Practice of Medicine > Medical Climatology > WB 710 Diseases of geographic areas
QX Parasitology > Insects. Other Parasites > QX 505 Diptera
WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
WA Public Health > WA 105 Epidemiology
Faculty: Department: Groups (2002 - 2012) > Vector Group
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ympev.2008.10.011
Depositing User: Users 183 not found.
Date Deposited: 10 Mar 2010 14:15
Last Modified: 06 Feb 2018 12:59
URI: http://archive.lstmed.ac.uk/id/eprint/358

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