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Interdomain lateral gene transfer of an essential ferrochelatase gene in human parasitic nematodes.

Wu, Bo, Novelli, Jacopo, Jiang, Daojun, Dailey, Harry A, Landmann, Frédéric, Ford, Louise, Taylor, Mark, Carlow, Clotilde K S, Kumar, Sanjay, Foster, Jeremy M and Slatko, Barton E (2013) 'Interdomain lateral gene transfer of an essential ferrochelatase gene in human parasitic nematodes.'. Proceedings of the National Academy of Sciences, Vol 110, Issue 19, pp. 7748-7753.

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Abstract

Lateral gene transfer events between bacteria and animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral gene transfer in animal parasitic nematodes. Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; however, the human filarial parasite Brugia malayi has acquired a bacterial gene encoding ferrochelatase (BmFeCH), the terminal step in heme biosynthesis. BmFeCH, encoded by a 9-exon gene, is a mitochondrial-targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies. Homologs have been identified in several filariae and a nonfilarial nematode. RNAi and ex vivo inhibitor experiments indicate that BmFeCH is essential for viability, validating it as a potential target for filariasis control.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 475 Genetic processes
QX Parasitology > Helminths. Annelida > QX 203 Nematoda
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 880 Filariasis and related conditions (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1073/pnas.1304049110
Depositing User: Mary Creegan
Date Deposited: 13 Aug 2014 09:04
Last Modified: 06 Feb 2018 13:07
URI: https://archive.lstmed.ac.uk/id/eprint/3840

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