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Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

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Lawson, Lovett, Emenyonu, Nanmdi, Abdurrahman, Saddiq T, Lawson, Juliana O, Uzoewulu, Gertrude N, Sogaolu, Olumide M, Ebisike, Juliana N, Parry, Christopher M, Yassin, Mohammed A. and Cuevas, Luis ORCID: https://orcid.org/0000-0002-6581-0587 (2013) 'Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria'. BMC Research Notes, Vol 6, : 215.

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Abstract

Background
This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.

Methods
This was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.

Results
527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.

Conclusions
There was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

Item Type: Article
Subjects: QV Pharmacology > QV 38 Drug action.
QW Microbiology and Immunology > Bacteria > QW 125 Actinibacteria, Actinomycetales.
QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified.
WF Respiratory System > Tuberculosis > WF 220 Diagnosis. Prognosis
WF Respiratory System > Tuberculosis > WF 360 Drug therapy
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1186/1756-0500-6-215
Depositing User: Martin Chapman
Date Deposited: 18 Nov 2014 10:25
Last Modified: 06 Feb 2018 13:07
URI: http://archive.lstmed.ac.uk/id/eprint/4526

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