LSTM Home > LSTM Research > LSTM Online Archive

Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung

Downloads

Downloads per month over past year

Wright, Adam, Bangert, Mathieu, Gritzfeld, Jenna, Ferreira, Daniela ORCID: https://orcid.org/0000-0001-7730-9477, Jambo, Kondwani, Wright, Angela D., Collins, Andrea ORCID: https://orcid.org/0000-0002-4094-1572 and Gordon, Stephen ORCID: https://orcid.org/0000-0001-6576-1116 (2013) 'Experimental Human Pneumococcal Carriage Augments IL-17A-dependent T-cell Defence of the Lung'. PLoS Pathogens, Vol 9, Issue 3, e1003274.

[img]
Preview
Text
Plos_Path_9_3_e1003274.pdf - Published Version
Available under License Creative Commons Attribution.

Download (580kB) | Preview

Abstract

Pneumococcal carriage is both immunising and a pre-requisite for mucosal and systemic disease. Murine models of pneumococcal colonisation show that IL-17A-secreting CD4+ T-cells (Th-17 cells) are essential for clearance of pneumococci from the nasopharynx. Pneumococcal-responding IL-17A-secreting CD4+ T-cells have not been described in the adult human lung and it is unknown whether they can be elicited by carriage and protect the lung from pneumococcal infection. We investigated the direct effect of experimental human pneumococcal nasal carriage (EHPC) on the frequency and phenotype of cognate CD4+ T-cells in broncho-alveolar lavage and blood using multi-parameter flow cytometry. We then examined whether they could augment ex vivo alveolar macrophage killing of pneumococci using an in vitro assay. We showed that human pneumococcal carriage leads to a 17.4-fold (p = 0.007) and 8-fold (p = 0.003) increase in the frequency of cognate IL-17A+ CD4+ T-cells in BAL and blood, respectively. The phenotype with the largest proportion were TNF+/IL-17A+ co-producing CD4+ memory T-cells (p<0.01); IFNγ+ CD4+ memory T-cells were not significantly increased following carriage. Pneumococci could stimulate large amounts of IL-17A protein from BAL cells in the absence of carriage but in the presence of cognate CD4+ memory T-cells, IL-17A protein levels were increased by a further 50%. Further to this we then show that alveolar macrophages, which express IL-17A receptors A and C, showed enhanced killing of opsonised pneumococci when stimulated with rhIL-17A (p = 0.013). Killing negatively correlated with RC (r = −0.9, p = 0.017) but not RA expression. We conclude that human pneumococcal carriage can increase the proportion of lung IL-17A-secreting CD4+ memory T-cells that may enhance innate cellular immunity against pathogenic challenge. These pathways may be utilised to enhance vaccine efficacy to protect the lung against pneumonia.

Item Type: Article
Subjects: QU Biochemistry > Cells and Genetics > QU 350 Cellular structures
WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 217 Pneumococcal infections
WF Respiratory System > WF 20 Research (General)
WF Respiratory System > Lungs > WF 600 Lungs
Faculty: Department: Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1371/journal.ppat.1003274
Depositing User: Lynn Roberts-Maloney
Date Deposited: 02 Dec 2014 13:41
Last Modified: 12 Oct 2018 08:56
URI: https://archive.lstmed.ac.uk/id/eprint/4607

Statistics

View details

Actions (login required)

Edit Item Edit Item