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PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

Pennington, Shaun ORCID: https://orcid.org/0000-0002-7160-6275, Thompson, Ameeka, Wright, Adam, Ferreira, Daniela ORCID: https://orcid.org/0000-0001-7730-9477, Wright, Angela, Faragher, Brian, Gordon, Stephen ORCID: https://orcid.org/0000-0001-6576-1116 and Gordon, M. A. (2012) 'PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination'. Gut, Vol 61, Issue Supp 2, A349-A350.

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Abstract

Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.

Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined by flow cytometric analysis for CD3+/CD8+ and CD3+/CD8− (CD4+) lymphocytes. Humoural IgA, IgM and IgG responses in blood were examined in relation to mucosal and peripheral cellular responses.

Results: Oral immunisation with Ty21a significantly increased the proportion of antigen-specific cytokine-producing CD8-positive (p<0.05) and CD8-negative (p<0.05) lymphocytes within the duodenal mucosa, but no specific response was seen in colon. CD8-negative lymphocytes within the duodenal mucosa adopted a significantly more poly-functional phenotype following vaccination, expressing 2 or 3 cytokines simultaneously, while in contrast antigen-specific cytokine-producing CD8-positive lymphocytes in the duodenal mucosa were mono-functional expressing a single cytokine. In blood, the proportion of antigen-specific cytokine-producing CD8-positive lymphocytes was increased (p<0.05) following oral vaccination, but there was no significant increase in cytokine-producing CD4-positive lymphocytes. Differences in functionality of antigen-specific cytokine responses were less marked in peripheral blood lymphocytes following vaccination.

Conclusion: These data show an antigen-specific response in human gut mucosal lymphocytes following oral vaccination, and directly demonstrate different immune functionality of CD8-positive compared to CD8-negative mucosal lymphocytes. These responses were more informative than surrogate measurements in peripheral blood lymphocytes. The absence of a detectable cognate response from the colon may indicate compartmentalisation of the gut mucosal response to the embryological mid-gut, where typhoid antigen is likely presented at immune inductive sites.

Item Type: Article
Additional Information: Poster abstract from Digestive Disorders Federation Meeting 17–20 June 2012, Liverpool
Subjects: QU Biochemistry > Cells and Genetics > QU 300 General works
QW Microbiology and Immunology > Immunotherapy and Hypersensitivity > QW 805 Vaccines. Antitoxins. Toxoids
WC Communicable Diseases > Infection. Bacterial Infections > Enteric Infections > WC 270 Typhoid fever
Digital Object Identifer (DOI): https://doi.org/10.1136/gutjnl-2012-302514d.130
Depositing User: Martin Chapman
Date Deposited: 05 Jan 2015 12:25
Last Modified: 23 Feb 2018 09:31
URI: https://archive.lstmed.ac.uk/id/eprint/4696

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