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Human cerebral malaria and Plasmodium falciparum genotypes in Malawi

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Milner, Danny A, Vareta, Jimmy, Valim, Clarissa, Montgomery, Jacqui, Daniels, Rachel F, Volkman, Sarah K, Neafsey, Daniel E, Park, Daniel J, Schaffner, Stephen F, Mahesh, Nira C, Barnes, Kayla G, Rosen, David M, Lukens, Amanda K, Van-Tyne, Daria, Wiegand, Roger C, Sabeti, Pardis C, Seydel, Karl B, Glover, Simon J, Kamiza, Steve, Molyneux, Malcolm E, Taylor, Terrie E and Wirth, Dyann F (2012) 'Human cerebral malaria and Plasmodium falciparum genotypes in Malawi'. Malaria Journal, Vol 11, e35.

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Abstract

Background

Cerebral malaria, a severe form of Plasmodium falciparum infection, is an important cause of mortality in sub-Saharan African children. A Taqman 24 Single Nucleotide Polymorphisms (SNP) molecular barcode assay was developed for use in laboratory parasites which estimates genotype number and identifies the predominant genotype.

Methods

The 24 SNP assay was used to determine predominant genotypes in blood and tissues from autopsy and clinical patients with cerebral malaria.

Results

Single genotypes were shared between the peripheral blood, the brain, and other tissues of cerebral malaria patients, while malaria-infected patients who died of non-malarial causes had mixed genetic signatures in tissues examined. Children with retinopathy-positive cerebral malaria had significantly less complex infections than those without retinopathy (OR = 3.7, 95% CI [1.51-9.10]).The complexity of infections significantly decreased over the malaria season in retinopathy-positive patients compared to retinopathy-negative patients.

Conclusions

Cerebral malaria patients harbour a single or small set of predominant parasites; patients with incidental parasitaemia sustain infections involving diverse genotypes. Limited diversity in the peripheral blood of cerebral malaria patients and correlation with tissues supports peripheral blood samples as appropriate for genome-wide association studies of parasite determinants of pathogenicity.

Item Type: Article
Additional Information: The electronic version of this article is the complete one and can be found online at: http://www.malariajournal.com/content/11/1/35
Subjects: QU Biochemistry > QU 26.5 Informatics. Automatic data processing. Computers
QU Biochemistry > Genetics > QU 470 Genetic structures
QU Biochemistry > Genetics > QU 550 Genetic techniques. PCR. Chromosome mapping
QX Parasitology > Protozoa > QX 135 Plasmodia
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
Faculty: Department: Groups (2002 - 2012) > Clinical Group
Groups (2002 - 2012) > Molecular & Biochemical Parasitology Group
Digital Object Identifer (DOI): https://doi.org/10.1186/1475-2875-11-35
Depositing User: Lynn Roberts-Maloney
Date Deposited: 27 Jan 2015 16:08
Last Modified: 06 Feb 2018 13:08
URI: http://archive.lstmed.ac.uk/id/eprint/4803

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