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A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.

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Meekums, Hayley, Hawash, Mohamed B F, Sparks, Alexandra M., Oviedo, Yisela, Sandoval, Carlos, Chico, Martha E, Stothard, Russell ORCID: https://orcid.org/0000-0002-9370-3420, Cooper, Philip J, Nejsum, Peter and Betson, Martha (2015) 'A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.'. Parasites & Vectors, Vol 8, e168.

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Abstract

BACKGROUND

Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador.

FINDINGS

Adult Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according to host species. In PCR-RFLP analysis of the ribosomal Internal Transcribed Spacer (ITS-2) and 18S DNA (59 pig worms and 82 human worms), nearly all Trichuris exhibited expected restriction patterns. However, two pig-derived worms showed a "heterozygous-type" ITS-2 pattern, with one also having a "heterozygous-type" 18S pattern. Phylogenetic analysis of the mitochondrial large ribosomal subunit partitioned worms by host species. Notably, some Ecuadorian T. suis clustered with porcine Trichuris from USA and Denmark and some with Chinese T. suis.

CONCLUSION

This is the first study in Latin America to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling.

Item Type: Article
Additional Information: The electronic version of this article is the complete one and can be found online at: http://www.parasitesandvectors.com/content/8/1/168
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QX Parasitology > Helminths. Annelida > QX 203 Nematoda
QX Parasitology > Helminths. Annelida > QX 207 Trichuroidea
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 950 Zoonoses (General)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1186/s13071-015-0782-9
Depositing User: Mary Creegan
Date Deposited: 09 Sep 2015 08:27
Last Modified: 06 Feb 2018 13:10
URI: https://archive.lstmed.ac.uk/id/eprint/5297

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