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Characterization of ascaris from Ecuador and Zanzibar

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Sparks, Alexandra M., Betson, M, Oviedo, G, Sandoval, C, Cooper, P J and Stothard, Russell ORCID: https://orcid.org/0000-0002-9370-3420 (2015) 'Characterization of ascaris from Ecuador and Zanzibar'. Journal of Helminthology, Vol 89, Issue 4, pp. 512-515.

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Abstract

To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis revealed the presence of A. lumbricoides in people and A. suum in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as A. suum. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species, A. suum being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar.

Item Type: Article
Subjects: QX Parasitology > Helminths. Annelida > QX 200 Helminths
QX Parasitology > Helminths. Annelida > QX 277 Ascaroidea
WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 870 Ascariasis
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1017/S0022149X14000431
Depositing User: Mary Creegan
Date Deposited: 15 Sep 2015 09:23
Last Modified: 06 Feb 2018 13:10
URI: http://archive.lstmed.ac.uk/id/eprint/5298

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