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Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

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Sikulu, Maggy T., Monkman, James, Dave, Keyur A., Hastie, Marcus L., Dale, Patricia E., Kitching, Roger L., Killeen, Gerry ORCID: https://orcid.org/0000-0002-8583-8739, Kay, Brian H., Gorman, Jeffry J. and Hugo, Leon E. (2015) 'Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi'. Data in Brief, Vol 4, pp. 461-467.

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Abstract

This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 460 Genomics. Proteomics
QU Biochemistry > Proteins. Amino Acids. Peptides > QU 55 Proteins
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1016/j.dib.2015.07.007
Depositing User: Carmel Bates
Date Deposited: 11 Sep 2015 08:39
Last Modified: 16 Sep 2019 10:12
URI: https://archive.lstmed.ac.uk/id/eprint/5304

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