LSTM Home > LSTM Research > LSTM Online Archive

Evaluation of human and mosquito based diagnostic tools for defining endpoints for elimination of Anopheles transmitted lymphatic filariasis in Ghana

Owusu, Irene Offei, de Souza, Dziedzom Komi, Anto, Francis, Wilson, Michael, Boakye, Daniel B, Bockarie, Moses and Gyapong, John (2015) 'Evaluation of human and mosquito based diagnostic tools for defining endpoints for elimination of Anopheles transmitted lymphatic filariasis in Ghana'. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol 109, Issue 10, pp. 628-635.

Full text not available from this repository.

Abstract

Background:
The decision to stop mass drug administration (MDA) and monitor recrudescence has to be made when endpoints for elimination of lymphatic filariasis (LF) have been achieved. Highly sensitive and specific diagnostic tools are required to do this. The main objective of this study was to determine most effective diagnostic tools for assessing interruption of LF transmission.

Methods:
The presence of filarial infection in blood and mosquito samples was determined using five diagnostic tools: Brugia malayi-14 (BM14) antibody detection ELISA, Onchocerca gibsoni antigen (Og4C3) based ELISA, PCR, immunochromatography (ICT) card test and blood smear. The study was carried out in two communities in the Central Region of Ghana.

Results:
OG4C3 was found to be the most sensitive test but ICT, the second most sensitive, was the most field applicable. PCR was found to be the most specific. Thirteen out of 30 pools of anopheles mosquitoes tested positive for the DNA of Wuchereria bancrofti.

Conclusions:
Very low antigen prevalence in primary school children indicates that MDA is working, so children born since the intervention was put in place are not getting infected. Inclusion of xenomonitoring in monitoring the effectiveness of MDA will give a better indication as to when transmission has been interrupted especially in areas where microfilaria prevalence is lower than 1%.

Evaluation of human and mosquito based diagnostic tools for defining endpoints for elimination of Anopheles transmitted lymphatic filariasis in Ghana (PDF Download Available). Available from: https://www.researchgate.net/publication/281861241_Evaluation_of_human_and_mosquito_based_diagnostic_tools_for_defining_endpoints_for_elimination_of_Anopheles_transmitted_lymphatic_filariasis_in_Ghana [accessed Jan 25, 2016].

Item Type: Article
Additional Information: Subjects: SM
Subjects: QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WA Public Health > WA 100 General works
WA Public Health > Health Administration and Organization > WA 540 National and state health administration
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1093/trstmh/trv070
Depositing User: Stacy Murtagh
Date Deposited: 25 Jan 2016 14:04
Last Modified: 06 Feb 2018 13:11
URI: http://archive.lstmed.ac.uk/id/eprint/5530

Statistics

View details

Actions (login required)

Edit Item Edit Item