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Pyriproxyfen is metabolized by P450s associated with pyrethroid resistance in An. gambiae.

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YuntaYanes, Cristina, Grisales, Nelson, Nász, Szilárd, Hemmings, Kay, Pignatelli, Patricia, Voice, Michael, Ranson, Hilary ORCID: https://orcid.org/0000-0003-2332-8247 and Paine, Mark ORCID: https://orcid.org/0000-0003-2061-7713 (2016) 'Pyriproxyfen is metabolized by P450s associated with pyrethroid resistance in An. gambiae.'. Insect biochemistry and molecular biology, Vol 78, pp. 50-57.

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Abstract

Pyrethroid resistance is widespread in the malaria vector Anopheles gambiae leading to concerns about the future efficacy of bednets with pyrethroids as the sole active ingredient. The incorporation of pyriproxyfen (PPF), a juvenile hormone analogue, into pyrethroid treated bednets is being trialed in Africa. Pyrethroid resistance is commonly associated with elevated levels of P450 expression including CYPs 6M2, 6P2, 6P3, 6P4, 6P5, 6Z2 and 9J5. Having expressed these P450s in E. coli we find all are capable of metabolizing PPF. Inhibition of these P450s by permethrin, deltamethrin and PPF was also examined. Deltamethrin and permethrin were moderate inhibitors (IC50 1-10 μM) of diethoxyfluorescein (DEF) activity for all P450s apart from CYP6Z2 (IC50 > 10 μM), while PPF displayed weaker inhibition of all P450s (IC50 > 10 μM) except CYP's 6Z2 and 6P2 (IC50 1-10 μM). We found evidence of low levels of cross resistance between PPF and other insecticide classes by comparing the efficacy of PPF in inhibiting metamorphosis and inducing female sterility in an insecticide susceptible strain of An. gambiae and a multiple resistant strain from Cote d'Ivoire.

Item Type: Article
Subjects: QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by)
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 750 Malaria
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
WH Hemic and Lymphatic Systems > Hematologic Diseases. Immunologic Factors. Blood Banks > WH 190 Hemoglobin and other hemeproteins. Porphyrins (Associated with hemoglobin)
Faculty: Department: Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1016/j.ibmb.2016.09.001
Depositing User: Carmel Bates
Date Deposited: 19 Sep 2016 13:28
Last Modified: 06 Sep 2017 01:02
URI: http://archive.lstmed.ac.uk/id/eprint/6147

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