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The last bastion? X-chromosome genotyping of Anopheles gambiae species-pair males from a hybrid zone reveals complex recombination within the major candidate ‘genomic island of speciation’

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Caputo, B., Pichler, V, Mancini, E, Pombi, M, Vicente, J.L., Dinis, J, Steen, Keith ORCID: https://orcid.org/0000-0002-8933-8643, Petrarca, V, Rodrigues, A, Pinto, J, della Torre, A. and Weetman, David ORCID: https://orcid.org/0000-0002-5820-1388 (2016) 'The last bastion? X-chromosome genotyping of Anopheles gambiae species-pair males from a hybrid zone reveals complex recombination within the major candidate ‘genomic island of speciation’'. Molecular Ecology, Vol 25, Issue 22, pp. 5719-5731.

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Abstract

Speciation with gene flow may be aided by reduced recombination helping to build linkage between genes involved in the early stages of reproductive isolation. Reduced recombination on chromosome-X has been implicated in speciation within the Anopheles gambiae complex, species of which represent the major Afrotropical malaria vectors. The most recently diverged, morphologically-indistinguishable, species-pair, An. gambiae and An. coluzzii, ubiquitously display a ‘genomic island of divergence’ spanning over 4Mb from chromosome-X centromere, which represents a particularly promising candidate region for reproductive isolation genes, in addition to containing the diagnostic markers used to distinguish the species. Very low recombination makes the island intractable for experimental recombination studies, but an extreme hybrid zone in Guinea Bissau offers the opportunity for natural investigation of X-island recombination. SNP-analysis of chromosome-X hemizygous males revealed: (i) strong divergence in the X-island despite a lack of autosomal divergence; (ii) individuals with multiple-recombinant genotypes, including likely double crossovers and localized gene conversion; (iii) recombination-driven discontinuity both within and between the molecular species markers, suggesting that the utility of the diagnostics is undermined under high hybridization. The largely-, but incompletely-protected nature of the X-centromeric genomic island is consistent with a primary candidate area for accumulation of adaptive variants driving speciation with gene flow, whilst permitting some selective shuffling and removal of genetic variation.

Item Type: Article
Subjects: QU Biochemistry > Genetics > QU 470 Genetic structures
QU Biochemistry > Genetics > QU 500 Genetic phenomena
QX Parasitology > Insects. Other Parasites > QX 515 Anopheles
QX Parasitology > Insects. Other Parasites > QX 650 Insect vectors
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI): https://doi.org/10.1111/mec.13840
Depositing User: Carmel Bates
Date Deposited: 29 Sep 2016 14:43
Last Modified: 22 Sep 2017 01:02
URI: http://archive.lstmed.ac.uk/id/eprint/6220

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