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Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis

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Tamarozzi, Francesca, Turner, Joseph ORCID: https://orcid.org/0000-0002-2185-5476, Pionnier, Nicolas ORCID: https://orcid.org/0000-0002-2379-4945, Midgley, Angela, Guimaraes, Ana, Johnston, Kelly, Edwards, Steven W and Taylor, Mark ORCID: https://orcid.org/0000-0003-3396-9275 (2016) 'Wolbachia endosymbionts induce neutrophil extracellular trap formation in human onchocerciasis'. Scientific Reports, Vol 6, p. 35559.

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Abstract

The endosymbiotic bacteria, Wolbachia, induce neutrophilic responses to the human helminth pathogen Onchocerca volvulus. The formation of Neutrophil Extracellular Traps (NETs), has been implicated in anti-microbial defence, but has not been identified in human helminth infection. Here, we demonstrate NETs formation in human onchocerciasis. Extracellular NETs and neutrophils were visualised around O. volvulus in nodules excised from untreated patients but not in nodules from patients treated with the anti-Wolbachia drug, doxycycline. Whole Wolbachia or microspheres coated with a synthetic Wolbachia lipopeptide (WoLP) of the major nematode Wolbachia TLR2/6 ligand, peptidoglycan associated lipoprotein, induced NETosis in human neutrophils in vitro. TLR6 dependency of Wolbachia and WoLP NETosis was demonstrated using purified neutrophils from TLR6 deficient mice. Thus, we demonstrate for the first time that NETosis occurs during natural human helminth infection and demonstrate a mechanism of NETosis induction via Wolbachia endobacteria and direct ligation of Wolbachia lipoprotein by neutrophil TLR2/6.

Item Type: Article
Subjects: QW Microbiology and Immunology > QW 51 Morphology and variability of microorganisms. Microbial genetics.
WA Public Health > Preventive Medicine > WA 110 Prevention and control of communicable diseases. Transmission of infectious diseases
WC Communicable Diseases > Tropical and Parasitic Diseases > WC 885 Onchocerciasis
WW Ophthalmology > Diseases. Color Perception > WW 160 Eye infections. Hypersensitivity diseases (General or not elsewhere classified)
Faculty: Department: Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI): https://doi.org/10.1038/srep35559
Depositing User: Jessica Jones
Date Deposited: 28 Oct 2016 15:38
Last Modified: 16 Sep 2019 09:01
URI: https://archive.lstmed.ac.uk/id/eprint/6319

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