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Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America

Edwards, Thomas, del Carmen Castillo Signor, Leticia, Williams, Chris, Larcher, Clement, Espinel, Mauricio, Theaker, Jane, Donis, Evelin, Cuevas, Luis and Adams, Emily (2017) 'Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America'. Diagnostic Microbiology and Infectious Disease, Vol 89, Issue 1, pp. 35-39.

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Abstract

Chikungunya was introduced into the Americas in 2015 causing a pandemic across the continent. Testing during the acute phase of infection relies on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary strains. A probit analysis established the 95% limit of detection as 19.6 copies per reaction. We compared the assay with a US Centers for Disease Control (CDC) chikungunya qRT-PCR as the reference standard. The assay had a sensitivity and specificity of 98.4% and 100% in 90 samples retrospectively collected in Guatemala. In a further 74 febrile samples prospectively collected in Ecuador and Guatemala the test had a sensitivity and specificity of 100% and 98.4%, respectively. Sequencing the nsp4 gene of the discordant positive sample indicated the presence of chikungunya RNA, and mismatches to the primer binding sites of the CDC assay.

Item Type: Article
Subjects: QW Microbiology and Immunology > Viruses > QW 160 Viruses (General). Virology
QX Parasitology > QX 20 Research (General)
QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes
WA Public Health > Health Problems of Special Population Groups > WA 395 Health in developing countries
Faculty: Department: Biological Sciences > Parasitology Department
Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI): https://doi.org/10.1016/j.diagmicrobio.2017.06.001
Depositing User: Stacy Murtagh
Date Deposited: 28 Jun 2017 11:35
Last Modified: 22 Sep 2017 15:09
URI: http://archive.lstmed.ac.uk/id/eprint/7259

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